Cargando…

Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline

The current studies were aimed at evaluating the efficacy of intranasal pentoxifylline (Ptx) pretreatment in protecting mesodopaminergic system and hippocampus from oxidative damage of lithium-pilocarpine induced status epilepticus (SE) and the involvement of nuclear factor erythroid 2-related facto...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Yunxiao, Yan, Wensheng, Fang, Hui, Zhang, Guoliang, Du, Yakun, Wang, Lei, Cui, Huixian, Shi, Geming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366206/
https://www.ncbi.nlm.nih.gov/pubmed/28386312
http://dx.doi.org/10.1155/2017/7908072
_version_ 1782517551440330752
author Kang, Yunxiao
Yan, Wensheng
Fang, Hui
Zhang, Guoliang
Du, Yakun
Wang, Lei
Cui, Huixian
Shi, Geming
author_facet Kang, Yunxiao
Yan, Wensheng
Fang, Hui
Zhang, Guoliang
Du, Yakun
Wang, Lei
Cui, Huixian
Shi, Geming
author_sort Kang, Yunxiao
collection PubMed
description The current studies were aimed at evaluating the efficacy of intranasal pentoxifylline (Ptx) pretreatment in protecting mesodopaminergic system and hippocampus from oxidative damage of lithium-pilocarpine induced status epilepticus (SE) and the involvement of nuclear factor erythroid 2-related factor 2- (Nrf2-) antioxidant response elements pathway. Pentoxifylline was administered to rats intranasally or intraperitoneally 30 minutes before inducing SE. Our results showed the impaired visuospatial memory, the defected mesodopaminergic system, and the oxidative damage and the transient activation of Nrf2 in SE rats. The transient activation of Nrf2 in SE rats was enhanced by Ptx pretreatment, which was followed by the upregulation of heme oxygenase-1 and NAD(P)H:quinone oxidoreductase-1. Ptx pretreatment to SE rats significantly suppressed the epileptic seizures, decreased the levels of lipid peroxide and malondialdehyde, and elevated the ratio of reduced glutathione/oxidized glutathione. Compared with intraperitoneal injection, intranasal Ptx delivery completely restored the visuospatial memory and the activity of mesodopaminergic system in SE rats. Intranasal administration of Ptx may hopefully become a noninvasive, painless, and easily administered option for epileptic patients.
format Online
Article
Text
id pubmed-5366206
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-53662062017-04-06 Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline Kang, Yunxiao Yan, Wensheng Fang, Hui Zhang, Guoliang Du, Yakun Wang, Lei Cui, Huixian Shi, Geming Oxid Med Cell Longev Research Article The current studies were aimed at evaluating the efficacy of intranasal pentoxifylline (Ptx) pretreatment in protecting mesodopaminergic system and hippocampus from oxidative damage of lithium-pilocarpine induced status epilepticus (SE) and the involvement of nuclear factor erythroid 2-related factor 2- (Nrf2-) antioxidant response elements pathway. Pentoxifylline was administered to rats intranasally or intraperitoneally 30 minutes before inducing SE. Our results showed the impaired visuospatial memory, the defected mesodopaminergic system, and the oxidative damage and the transient activation of Nrf2 in SE rats. The transient activation of Nrf2 in SE rats was enhanced by Ptx pretreatment, which was followed by the upregulation of heme oxygenase-1 and NAD(P)H:quinone oxidoreductase-1. Ptx pretreatment to SE rats significantly suppressed the epileptic seizures, decreased the levels of lipid peroxide and malondialdehyde, and elevated the ratio of reduced glutathione/oxidized glutathione. Compared with intraperitoneal injection, intranasal Ptx delivery completely restored the visuospatial memory and the activity of mesodopaminergic system in SE rats. Intranasal administration of Ptx may hopefully become a noninvasive, painless, and easily administered option for epileptic patients. Hindawi 2017 2017-03-12 /pmc/articles/PMC5366206/ /pubmed/28386312 http://dx.doi.org/10.1155/2017/7908072 Text en Copyright © 2017 Yunxiao Kang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, Yunxiao
Yan, Wensheng
Fang, Hui
Zhang, Guoliang
Du, Yakun
Wang, Lei
Cui, Huixian
Shi, Geming
Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline
title Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline
title_full Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline
title_fullStr Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline
title_full_unstemmed Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline
title_short Alleviation of Oxidative Damage and Involvement of Nrf2-ARE Pathway in Mesodopaminergic System and Hippocampus of Status Epilepticus Rats Pretreated by Intranasal Pentoxifylline
title_sort alleviation of oxidative damage and involvement of nrf2-are pathway in mesodopaminergic system and hippocampus of status epilepticus rats pretreated by intranasal pentoxifylline
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366206/
https://www.ncbi.nlm.nih.gov/pubmed/28386312
http://dx.doi.org/10.1155/2017/7908072
work_keys_str_mv AT kangyunxiao alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline
AT yanwensheng alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline
AT fanghui alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline
AT zhangguoliang alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline
AT duyakun alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline
AT wanglei alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline
AT cuihuixian alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline
AT shigeming alleviationofoxidativedamageandinvolvementofnrf2arepathwayinmesodopaminergicsystemandhippocampusofstatusepilepticusratspretreatedbyintranasalpentoxifylline