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Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia

BACKGROUND: Nepeta dschuparensis Bornm (NP) is used as a medicinal herb in Iran. In traditional medicine, this herb is extensively employed for curing ailments such as cardiovascular diseases. NP has antioxidant and anti-inflammatory properties. This project examined the effects of the NP extract on...

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Autores principales: Nia, Alireza Mousavi, Kalantaripour, Taj Pari, Basiri, Mohsen, Vafaee, Farzaneh, Asadi-Shekaari, Majid, Eslami, Azam, Zadeh, Fatemeh Darvish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Journal of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366366/
https://www.ncbi.nlm.nih.gov/pubmed/28360444
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author Nia, Alireza Mousavi
Kalantaripour, Taj Pari
Basiri, Mohsen
Vafaee, Farzaneh
Asadi-Shekaari, Majid
Eslami, Azam
Zadeh, Fatemeh Darvish
author_facet Nia, Alireza Mousavi
Kalantaripour, Taj Pari
Basiri, Mohsen
Vafaee, Farzaneh
Asadi-Shekaari, Majid
Eslami, Azam
Zadeh, Fatemeh Darvish
author_sort Nia, Alireza Mousavi
collection PubMed
description BACKGROUND: Nepeta dschuparensis Bornm (NP) is used as a medicinal herb in Iran. In traditional medicine, this herb is extensively employed for curing ailments such as cardiovascular diseases. NP has antioxidant and anti-inflammatory properties. This project examined the effects of the NP extract on cyclooxygenase-2 (COX-2) and interleukin-1β (IL-1β) protein levels and its efficacy in neuroprotection in a cerebral ischemia-reperfusion model. METHODS: Twenty-six male rats were randomly divided into 3 groups: 1) sham (n=6): no middle cerebral artery occlusion (MCAO) procedure, 2) control (n=10): MCAO procedure and treatment with normal saline, and 3) NP extract (n=10): MCAO procedure and treatment with the NP extract (20 mg/kg, i.p.) at the beginning of reperfusion. To examine the injury caused by cerebral ischemia, we measured motor coordination and the infarct area using the rotarod test and triphenyl tetrazolium chloride staining, respectively. IL-1β and COX-2 protein levels, as inflammatory markers, were measured by immunoblotting assay. The statistical analyses were performed using SPSS, version 16, and the data are expressed as means±SEMs. Statistical difference was evaluated using the one-way ANOVA, followed by the post hoc LSD test (P<0.01). RESULTS: Treatment with the NP extract significantly diminished the infarct volume and alleviated the motor coordination disorder induced by cerebral ischemia. The NP extract administration significantly attenuated the increase in IL-1β and COX-2 protein levels too (P<0.01). CONCLUSION: The beneficial effects of the NP extract are related to its ability to decrease the levels of IL-1β and COX-2.
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spelling pubmed-53663662017-03-30 Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia Nia, Alireza Mousavi Kalantaripour, Taj Pari Basiri, Mohsen Vafaee, Farzaneh Asadi-Shekaari, Majid Eslami, Azam Zadeh, Fatemeh Darvish Iran J Med Sci Original Article BACKGROUND: Nepeta dschuparensis Bornm (NP) is used as a medicinal herb in Iran. In traditional medicine, this herb is extensively employed for curing ailments such as cardiovascular diseases. NP has antioxidant and anti-inflammatory properties. This project examined the effects of the NP extract on cyclooxygenase-2 (COX-2) and interleukin-1β (IL-1β) protein levels and its efficacy in neuroprotection in a cerebral ischemia-reperfusion model. METHODS: Twenty-six male rats were randomly divided into 3 groups: 1) sham (n=6): no middle cerebral artery occlusion (MCAO) procedure, 2) control (n=10): MCAO procedure and treatment with normal saline, and 3) NP extract (n=10): MCAO procedure and treatment with the NP extract (20 mg/kg, i.p.) at the beginning of reperfusion. To examine the injury caused by cerebral ischemia, we measured motor coordination and the infarct area using the rotarod test and triphenyl tetrazolium chloride staining, respectively. IL-1β and COX-2 protein levels, as inflammatory markers, were measured by immunoblotting assay. The statistical analyses were performed using SPSS, version 16, and the data are expressed as means±SEMs. Statistical difference was evaluated using the one-way ANOVA, followed by the post hoc LSD test (P<0.01). RESULTS: Treatment with the NP extract significantly diminished the infarct volume and alleviated the motor coordination disorder induced by cerebral ischemia. The NP extract administration significantly attenuated the increase in IL-1β and COX-2 protein levels too (P<0.01). CONCLUSION: The beneficial effects of the NP extract are related to its ability to decrease the levels of IL-1β and COX-2. Iranian Journal of Medical Sciences 2017-03 /pmc/articles/PMC5366366/ /pubmed/28360444 Text en Copyright: © Iranian Journal of Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Nia, Alireza Mousavi
Kalantaripour, Taj Pari
Basiri, Mohsen
Vafaee, Farzaneh
Asadi-Shekaari, Majid
Eslami, Azam
Zadeh, Fatemeh Darvish
Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia
title Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia
title_full Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia
title_fullStr Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia
title_full_unstemmed Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia
title_short Nepeta Dschuparensis Bornm Extract Moderates COX-2 and IL-1β Proteins in a Rat Model of Cerebral Ischemia
title_sort nepeta dschuparensis bornm extract moderates cox-2 and il-1β proteins in a rat model of cerebral ischemia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366366/
https://www.ncbi.nlm.nih.gov/pubmed/28360444
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