Cargando…

Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model

No studies have examined the transplantation of a bone marrow aspirate (BMA) containing mesenchymal stem cells (MSCs) combined with human parathyroid hormone 1-34 (hPTH1-34) administration. Therefore, we evaluated the feasibility and efficacy of autologous BMA transplantation combined with hPHT1-34...

Descripción completa

Detalles Bibliográficos
Autores principales: Makihara, Takeshi, Yoshioka, Tomokazu, Sugaya, Hisashi, Aoto, Katsuya, Wada, Hiroshi, Uemura, Kenta, Tanaka, Kenta, Akaogi, Hiroshi, Yamazaki, Masashi, Mishima, Hajime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366770/
https://www.ncbi.nlm.nih.gov/pubmed/28386485
http://dx.doi.org/10.1155/2017/2484689
_version_ 1782517651289931776
author Makihara, Takeshi
Yoshioka, Tomokazu
Sugaya, Hisashi
Aoto, Katsuya
Wada, Hiroshi
Uemura, Kenta
Tanaka, Kenta
Akaogi, Hiroshi
Yamazaki, Masashi
Mishima, Hajime
author_facet Makihara, Takeshi
Yoshioka, Tomokazu
Sugaya, Hisashi
Aoto, Katsuya
Wada, Hiroshi
Uemura, Kenta
Tanaka, Kenta
Akaogi, Hiroshi
Yamazaki, Masashi
Mishima, Hajime
author_sort Makihara, Takeshi
collection PubMed
description No studies have examined the transplantation of a bone marrow aspirate (BMA) containing mesenchymal stem cells (MSCs) combined with human parathyroid hormone 1-34 (hPTH1-34) administration. Therefore, we evaluated the feasibility and efficacy of autologous BMA transplantation combined with hPHT1-34 administration in a bone necrosis model. The metatarsal bones of rabbits were necrotized using liquid nitrogen, and the rabbits received a BMA transplantation or saline injection followed by hPTH1-34 (30 μg/kg) or saline administration three times per week (n = 3-4 per group). The rabbits were euthanized at 12 weeks after the initiation of treatment. No systemic adverse effects or local neoplastic lesions were observed. Importantly, the rabbits in the BMA transplantation plus hPTH1-34 group showed the highest bone volumes and histological scores of new bone. These data confirmed the feasibility of BMA transplantation combined with hPTH1-34, at least during the experimental period. The observed efficacy may be explained by a synergistic effect from the stimulation of MSC differentiation to osteoblasts with hPTH1-34-mediated suppression of apoptosis in osteoblasts. These results indicate the promising potential for BMA transplantation combined with hPTH1-34 administration in bone necrosis treatment. Longer term experiments are needed to confirm the safety of this therapeutic strategy.
format Online
Article
Text
id pubmed-5366770
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-53667702017-04-06 Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model Makihara, Takeshi Yoshioka, Tomokazu Sugaya, Hisashi Aoto, Katsuya Wada, Hiroshi Uemura, Kenta Tanaka, Kenta Akaogi, Hiroshi Yamazaki, Masashi Mishima, Hajime Bone Marrow Res Research Article No studies have examined the transplantation of a bone marrow aspirate (BMA) containing mesenchymal stem cells (MSCs) combined with human parathyroid hormone 1-34 (hPTH1-34) administration. Therefore, we evaluated the feasibility and efficacy of autologous BMA transplantation combined with hPHT1-34 administration in a bone necrosis model. The metatarsal bones of rabbits were necrotized using liquid nitrogen, and the rabbits received a BMA transplantation or saline injection followed by hPTH1-34 (30 μg/kg) or saline administration three times per week (n = 3-4 per group). The rabbits were euthanized at 12 weeks after the initiation of treatment. No systemic adverse effects or local neoplastic lesions were observed. Importantly, the rabbits in the BMA transplantation plus hPTH1-34 group showed the highest bone volumes and histological scores of new bone. These data confirmed the feasibility of BMA transplantation combined with hPTH1-34, at least during the experimental period. The observed efficacy may be explained by a synergistic effect from the stimulation of MSC differentiation to osteoblasts with hPTH1-34-mediated suppression of apoptosis in osteoblasts. These results indicate the promising potential for BMA transplantation combined with hPTH1-34 administration in bone necrosis treatment. Longer term experiments are needed to confirm the safety of this therapeutic strategy. Hindawi 2017 2017-03-13 /pmc/articles/PMC5366770/ /pubmed/28386485 http://dx.doi.org/10.1155/2017/2484689 Text en Copyright © 2017 Takeshi Makihara et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Makihara, Takeshi
Yoshioka, Tomokazu
Sugaya, Hisashi
Aoto, Katsuya
Wada, Hiroshi
Uemura, Kenta
Tanaka, Kenta
Akaogi, Hiroshi
Yamazaki, Masashi
Mishima, Hajime
Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model
title Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model
title_full Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model
title_fullStr Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model
title_full_unstemmed Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model
title_short Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model
title_sort feasibility and efficacy of autologous bone marrow aspirate transplantation combined with human parathyroid hormone 1-34 administration to treat osteonecrosis in a rabbit model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366770/
https://www.ncbi.nlm.nih.gov/pubmed/28386485
http://dx.doi.org/10.1155/2017/2484689
work_keys_str_mv AT makiharatakeshi feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT yoshiokatomokazu feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT sugayahisashi feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT aotokatsuya feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT wadahiroshi feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT uemurakenta feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT tanakakenta feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT akaogihiroshi feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT yamazakimasashi feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel
AT mishimahajime feasibilityandefficacyofautologousbonemarrowaspiratetransplantationcombinedwithhumanparathyroidhormone134administrationtotreatosteonecrosisinarabbitmodel