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C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells

Human embryonic stem cells (hESCs) are a unique population of cells defined by their capacity for self-renewal and pluripotency. Here, we identified a previously uncharacterized gene in hESCs, C9ORF135, which is sharply downregulated during gastrulation and gametogenesis, along with the pluripotency...

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Autores principales: Zhou, Shixin, Liu, Yinan, Ma, Yumin, Zhang, Xiaoyan, Li, Yang, Wen, Jinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366912/
https://www.ncbi.nlm.nih.gov/pubmed/28345668
http://dx.doi.org/10.1038/srep45311
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author Zhou, Shixin
Liu, Yinan
Ma, Yumin
Zhang, Xiaoyan
Li, Yang
Wen, Jinhua
author_facet Zhou, Shixin
Liu, Yinan
Ma, Yumin
Zhang, Xiaoyan
Li, Yang
Wen, Jinhua
author_sort Zhou, Shixin
collection PubMed
description Human embryonic stem cells (hESCs) are a unique population of cells defined by their capacity for self-renewal and pluripotency. Here, we identified a previously uncharacterized gene in hESCs, C9ORF135, which is sharply downregulated during gastrulation and gametogenesis, along with the pluripotency factors OCT4, SOX2, and NANOG. Human ESCs express two C9ORF135 isoforms, the longer of which encodes a membrane-associated protein, as determined by immunostaining and western blotting of fractionated cell lysates. Moreover, the results of chromatin immunoprecipitation (ChIP), mass spectrometry (MS), and co-immunoprecipitation (co-IP) analyses demonstrated that C9ORF135 expression is regulated by OCT4 and SOX2 and that C9ORF135 interacts with non-muscle myosin IIA and myosin IIB. Collectively, these data indicated that C9ORF135 encodes a membrane-associated protein that may serve as a surface marker for undifferentiated hESCs.
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spelling pubmed-53669122017-03-28 C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells Zhou, Shixin Liu, Yinan Ma, Yumin Zhang, Xiaoyan Li, Yang Wen, Jinhua Sci Rep Article Human embryonic stem cells (hESCs) are a unique population of cells defined by their capacity for self-renewal and pluripotency. Here, we identified a previously uncharacterized gene in hESCs, C9ORF135, which is sharply downregulated during gastrulation and gametogenesis, along with the pluripotency factors OCT4, SOX2, and NANOG. Human ESCs express two C9ORF135 isoforms, the longer of which encodes a membrane-associated protein, as determined by immunostaining and western blotting of fractionated cell lysates. Moreover, the results of chromatin immunoprecipitation (ChIP), mass spectrometry (MS), and co-immunoprecipitation (co-IP) analyses demonstrated that C9ORF135 expression is regulated by OCT4 and SOX2 and that C9ORF135 interacts with non-muscle myosin IIA and myosin IIB. Collectively, these data indicated that C9ORF135 encodes a membrane-associated protein that may serve as a surface marker for undifferentiated hESCs. Nature Publishing Group 2017-03-27 /pmc/articles/PMC5366912/ /pubmed/28345668 http://dx.doi.org/10.1038/srep45311 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhou, Shixin
Liu, Yinan
Ma, Yumin
Zhang, Xiaoyan
Li, Yang
Wen, Jinhua
C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells
title C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells
title_full C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells
title_fullStr C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells
title_full_unstemmed C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells
title_short C9ORF135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells
title_sort c9orf135 encodes a membrane protein whose expression is related to pluripotency in human embryonic stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366912/
https://www.ncbi.nlm.nih.gov/pubmed/28345668
http://dx.doi.org/10.1038/srep45311
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