Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

We assayed Zika virus–infected mosquitoes stored at room temperature for <30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <10 days, and virus RNA was detected in samples held for 30 days.

Detalles Bibliográficos
Autores principales: Burkhalter, Kristen L., Savage, Harry M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2017
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367413/
https://www.ncbi.nlm.nih.gov/pubmed/28075325
http://dx.doi.org/10.3201/eid2304.161772
_version_ 1782517769070182400
author Burkhalter, Kristen L.
Savage, Harry M.
author_facet Burkhalter, Kristen L.
Savage, Harry M.
author_sort Burkhalter, Kristen L.
collection PubMed
description We assayed Zika virus–infected mosquitoes stored at room temperature for <30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <10 days, and virus RNA was detected in samples held for 30 days.
format Online
Article
Text
id pubmed-5367413
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-53674132017-04-07 Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay Burkhalter, Kristen L. Savage, Harry M. Emerg Infect Dis Dispatch We assayed Zika virus–infected mosquitoes stored at room temperature for <30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <10 days, and virus RNA was detected in samples held for 30 days. Centers for Disease Control and Prevention 2017-04 /pmc/articles/PMC5367413/ /pubmed/28075325 http://dx.doi.org/10.3201/eid2304.161772 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Burkhalter, Kristen L.
Savage, Harry M.
Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
title Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
title_full Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
title_fullStr Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
title_full_unstemmed Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
title_short Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
title_sort detection of zika virus in desiccated mosquitoes by real-time reverse transcription pcr and plaque assay
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367413/
https://www.ncbi.nlm.nih.gov/pubmed/28075325
http://dx.doi.org/10.3201/eid2304.161772
work_keys_str_mv AT burkhalterkristenl detectionofzikavirusindesiccatedmosquitoesbyrealtimereversetranscriptionpcrandplaqueassay
AT savageharrym detectionofzikavirusindesiccatedmosquitoesbyrealtimereversetranscriptionpcrandplaqueassay

Ejemplares similares