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Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota

Subclinical chronic inflammation (SCI) is associated with impaired animal growth. Previous work has demonstrated that olive-derived plant bioactives exhibit anti-inflammatory properties that could possibly counteract the growth-depressing effects of SCI. To test this hypothesis and define the underl...

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Autores principales: Liehr, Martin, Mereu, Alessandro, Pastor, Jose Javier, Quintela, Jose Carlos, Staats, Stefanie, Rimbach, Gerald, Ipharraguerre, Ignacio Rodolfo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367713/
https://www.ncbi.nlm.nih.gov/pubmed/28346507
http://dx.doi.org/10.1371/journal.pone.0174239
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author Liehr, Martin
Mereu, Alessandro
Pastor, Jose Javier
Quintela, Jose Carlos
Staats, Stefanie
Rimbach, Gerald
Ipharraguerre, Ignacio Rodolfo
author_facet Liehr, Martin
Mereu, Alessandro
Pastor, Jose Javier
Quintela, Jose Carlos
Staats, Stefanie
Rimbach, Gerald
Ipharraguerre, Ignacio Rodolfo
author_sort Liehr, Martin
collection PubMed
description Subclinical chronic inflammation (SCI) is associated with impaired animal growth. Previous work has demonstrated that olive-derived plant bioactives exhibit anti-inflammatory properties that could possibly counteract the growth-depressing effects of SCI. To test this hypothesis and define the underlying mechanism, we conducted a 30-day study in which piglets fed an olive-oil bioactive extract (OBE) and their control counterparts (C+) were injected repeatedly during the last 10 days of the study with increasing doses of Escherichia coli lipopolysaccharides (LPS) to induce SCI. A third group of piglets remained untreated throughout the study and served as a negative control (C-). In C+ pigs, SCI increased the circulating concentration of interleukin 1 beta (p < 0.001) and decreased feed ingestion (p < 0.05) and weight gain (p < 0.05). These responses were not observed in OBE animals. Although intestinal inflammation and colonic microbial ecology was not altered by treatments, OBE enhanced ileal mRNA abundance of tight and adherens junctional proteins (p < 0.05) and plasma recovery of mannitol (p < 0.05) compared with C+ and C-. In line with these findings, OBE improved transepithelial electrical resistance (p < 0.01) in TNF-α-challenged Caco-2/TC-7 cells, and repressed the production of inflammatory cytokines (p < 0.05) in LPS-stimulated macrophages. In summary, this work demonstrates that OBE attenuates the suppressing effect of SCI on animal growth through a mechanism that appears to involve improvements in intestinal integrity unrelated to alterations in gut microbial ecology and function.
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spelling pubmed-53677132017-04-06 Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota Liehr, Martin Mereu, Alessandro Pastor, Jose Javier Quintela, Jose Carlos Staats, Stefanie Rimbach, Gerald Ipharraguerre, Ignacio Rodolfo PLoS One Research Article Subclinical chronic inflammation (SCI) is associated with impaired animal growth. Previous work has demonstrated that olive-derived plant bioactives exhibit anti-inflammatory properties that could possibly counteract the growth-depressing effects of SCI. To test this hypothesis and define the underlying mechanism, we conducted a 30-day study in which piglets fed an olive-oil bioactive extract (OBE) and their control counterparts (C+) were injected repeatedly during the last 10 days of the study with increasing doses of Escherichia coli lipopolysaccharides (LPS) to induce SCI. A third group of piglets remained untreated throughout the study and served as a negative control (C-). In C+ pigs, SCI increased the circulating concentration of interleukin 1 beta (p < 0.001) and decreased feed ingestion (p < 0.05) and weight gain (p < 0.05). These responses were not observed in OBE animals. Although intestinal inflammation and colonic microbial ecology was not altered by treatments, OBE enhanced ileal mRNA abundance of tight and adherens junctional proteins (p < 0.05) and plasma recovery of mannitol (p < 0.05) compared with C+ and C-. In line with these findings, OBE improved transepithelial electrical resistance (p < 0.01) in TNF-α-challenged Caco-2/TC-7 cells, and repressed the production of inflammatory cytokines (p < 0.05) in LPS-stimulated macrophages. In summary, this work demonstrates that OBE attenuates the suppressing effect of SCI on animal growth through a mechanism that appears to involve improvements in intestinal integrity unrelated to alterations in gut microbial ecology and function. Public Library of Science 2017-03-27 /pmc/articles/PMC5367713/ /pubmed/28346507 http://dx.doi.org/10.1371/journal.pone.0174239 Text en © 2017 Liehr et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liehr, Martin
Mereu, Alessandro
Pastor, Jose Javier
Quintela, Jose Carlos
Staats, Stefanie
Rimbach, Gerald
Ipharraguerre, Ignacio Rodolfo
Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota
title Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota
title_full Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota
title_fullStr Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota
title_full_unstemmed Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota
title_short Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota
title_sort olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367713/
https://www.ncbi.nlm.nih.gov/pubmed/28346507
http://dx.doi.org/10.1371/journal.pone.0174239
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