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Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells

BACKGROUND: Hypoxic conditions play roles in functioning of human tendon-derived stem cells (hTSCs). The goal of this study was to investigate the effect of various hypoxic conditions in self-renewal capacity and differentiation of TSCs. MATERIAL/METHODS: hTSCs was obtain from supraspinatus tendon d...

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Detalles Bibliográficos
Autores principales: Yu, Yang, Lin, Lixiang, Zhou, Yifei, Lu, Xiaolang, Shao, Xiwen, Lin, Chuanlu, Yu, Kehe, Zhang, Xiaolei, Hong, Jianjun, Chen, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367841/
https://www.ncbi.nlm.nih.gov/pubmed/28302994
http://dx.doi.org/10.12659/MSM.903892
Descripción
Sumario:BACKGROUND: Hypoxic conditions play roles in functioning of human tendon-derived stem cells (hTSCs). The goal of this study was to investigate the effect of various hypoxic conditions in self-renewal capacity and differentiation of TSCs. MATERIAL/METHODS: hTSCs was obtain from supraspinatus tendon donors. Colony formation and cell proliferation assay were used to assess the self-renewal of hTSCs. qRT-PCT and Western blot analysis were used to examine stemness and multi-differentiation potential of hTSCs. RESULTS: We found that culturing at 5% O(2) is more beneficial for the self-renewal of hTSCs than the other 3 culture conditions, with larger colony size and numbers. The proliferation of hTSCs in 5%, 10%, and 20% O(2) cultures increased after seeding. The number of cells in the 5% O(2) condition was higher than that in other culture; however, self-renewal capacity of hTSCs in 0.5% O(2) was inhibited. The expression levels of stem cell markers, including NS, Nanog, Oct-4, and SSEA-4, were highest in 0.5% O(2) culture. Furthermore, hTSCs cultured in 20% O(2) exhibited significantly higher expression of the 3 markers (PPAR-γ, Sox-9, and Runx-2). CONCLUSIONS: Hypoxic condition of culture encouraged self-renewal capacity of hTSCs, but inhibited their multi-differentiation potential, compared to normoxic condition of culture. Moreover, excessively low oxygen concentration impaired the capacity of hTSCs.