Cargando…
Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage
The molecular characterization of 218 GBS isolates recovered from neonatal invasive infections in Portugal in 2005–2015 revealed the existence of a small number of genetically distinct lineages that were present over a significant time-span. Serotypes III and Ia were dominant in the population, toge...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368217/ https://www.ncbi.nlm.nih.gov/pubmed/28400757 http://dx.doi.org/10.3389/fmicb.2017.00499 |
_version_ | 1782517884172369920 |
---|---|
author | Martins, Elisabete R. Pedroso-Roussado, Cristiano Melo-Cristino, José Ramirez, Mário |
author_facet | Martins, Elisabete R. Pedroso-Roussado, Cristiano Melo-Cristino, José Ramirez, Mário |
author_sort | Martins, Elisabete R. |
collection | PubMed |
description | The molecular characterization of 218 GBS isolates recovered from neonatal invasive infections in Portugal in 2005–2015 revealed the existence of a small number of genetically distinct lineages that were present over a significant time-span. Serotypes III and Ia were dominant in the population, together accounting for >80% of the isolates. Clonal complex 17 included 50% of all isolates, highlighting the importance of the hypervirulent genetic lineage represented by serotype III ST17/rib/PI-1+PI-2b. Serotype Ia was represented mainly by ST23, previously reported as dominant among invasive disease in non-pregnant adults in Portugal, but also by ST24, showing an increased frequency among late-onset disease. Overall erythromycin resistance was 16%, increasing during the study period (p < 0.001). Macrolide resistance was overrepresented among CC1 and CC19 isolates (p < 0.001 and p = 0.008, respectively). While representatives of the hypervirulent CC17 lineage were mostly susceptible to macrolides, we identified for the first time in Europe a recently emerging sublineage characterized by the loss of PI-1 (CC17/PI-2b), simultaneously resistant to macrolides, lincosamides, and tetracycline, also exhibiting high-level resistance to streptomycin and kanamycin. The stability and dominance of CC17 among neonatal invasive infections in the past decades indicates that it is extremely well adapted to its niche; however emerging resistance in this genetic background may have significant implications for the prevention and management of GBS disease. |
format | Online Article Text |
id | pubmed-5368217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53682172017-04-11 Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage Martins, Elisabete R. Pedroso-Roussado, Cristiano Melo-Cristino, José Ramirez, Mário Front Microbiol Microbiology The molecular characterization of 218 GBS isolates recovered from neonatal invasive infections in Portugal in 2005–2015 revealed the existence of a small number of genetically distinct lineages that were present over a significant time-span. Serotypes III and Ia were dominant in the population, together accounting for >80% of the isolates. Clonal complex 17 included 50% of all isolates, highlighting the importance of the hypervirulent genetic lineage represented by serotype III ST17/rib/PI-1+PI-2b. Serotype Ia was represented mainly by ST23, previously reported as dominant among invasive disease in non-pregnant adults in Portugal, but also by ST24, showing an increased frequency among late-onset disease. Overall erythromycin resistance was 16%, increasing during the study period (p < 0.001). Macrolide resistance was overrepresented among CC1 and CC19 isolates (p < 0.001 and p = 0.008, respectively). While representatives of the hypervirulent CC17 lineage were mostly susceptible to macrolides, we identified for the first time in Europe a recently emerging sublineage characterized by the loss of PI-1 (CC17/PI-2b), simultaneously resistant to macrolides, lincosamides, and tetracycline, also exhibiting high-level resistance to streptomycin and kanamycin. The stability and dominance of CC17 among neonatal invasive infections in the past decades indicates that it is extremely well adapted to its niche; however emerging resistance in this genetic background may have significant implications for the prevention and management of GBS disease. Frontiers Media S.A. 2017-03-28 /pmc/articles/PMC5368217/ /pubmed/28400757 http://dx.doi.org/10.3389/fmicb.2017.00499 Text en Copyright © 2017 Martins, Pedroso-Roussado, Melo-Cristino, Ramirez and The Portuguese Group for the Study of Streptococcal Infections. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Martins, Elisabete R. Pedroso-Roussado, Cristiano Melo-Cristino, José Ramirez, Mário Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage |
title | Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage |
title_full | Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage |
title_fullStr | Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage |
title_full_unstemmed | Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage |
title_short | Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005–2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage |
title_sort | streptococcus agalactiae causing neonatal infections in portugal (2005–2015): diversification and emergence of a cc17/pi-2b multidrug resistant sublineage |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368217/ https://www.ncbi.nlm.nih.gov/pubmed/28400757 http://dx.doi.org/10.3389/fmicb.2017.00499 |
work_keys_str_mv | AT martinselisabeter streptococcusagalactiaecausingneonatalinfectionsinportugal20052015diversificationandemergenceofacc17pi2bmultidrugresistantsublineage AT pedrosoroussadocristiano streptococcusagalactiaecausingneonatalinfectionsinportugal20052015diversificationandemergenceofacc17pi2bmultidrugresistantsublineage AT melocristinojose streptococcusagalactiaecausingneonatalinfectionsinportugal20052015diversificationandemergenceofacc17pi2bmultidrugresistantsublineage AT ramirezmario streptococcusagalactiaecausingneonatalinfectionsinportugal20052015diversificationandemergenceofacc17pi2bmultidrugresistantsublineage AT streptococcusagalactiaecausingneonatalinfectionsinportugal20052015diversificationandemergenceofacc17pi2bmultidrugresistantsublineage |