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Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame

Osmotin-like proteins (OLPs), of PR-5 family, mediate defense against abiotic, and biotic stresses in plants. Overexpression in sesame of an OLP gene (SindOLP), enhanced tolerance against drought, salinity, oxidative stress, and the charcoal rot pathogen. SindOLP was expressed in all parts and local...

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Autores principales: Chowdhury, Supriyo, Basu, Arpita, Kundu, Surekha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368222/
https://www.ncbi.nlm.nih.gov/pubmed/28400780
http://dx.doi.org/10.3389/fpls.2017.00410
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author Chowdhury, Supriyo
Basu, Arpita
Kundu, Surekha
author_facet Chowdhury, Supriyo
Basu, Arpita
Kundu, Surekha
author_sort Chowdhury, Supriyo
collection PubMed
description Osmotin-like proteins (OLPs), of PR-5 family, mediate defense against abiotic, and biotic stresses in plants. Overexpression in sesame of an OLP gene (SindOLP), enhanced tolerance against drought, salinity, oxidative stress, and the charcoal rot pathogen. SindOLP was expressed in all parts and localized to the cytosol. The transgenic plants recovered after prolonged drought and salinity stress, showing less electrolyte leakage, more water content, longer roots, and smaller stomatal aperture compared to control plants. There was an increase in osmolytes, ROS-scavenging enzymes, chlorophyll content, proline, secondary metabolites, and reduced lipid peroxidation in the transgenic sesame under multiple stresses. The OLP gene imparted increased tolerance through the increased expression of three genes coding for ROS scavenging enzymes and five defense-related marker genes functioning in the JA/ET and SA pathways, namely Si-Apetala2, Si-Ethylene-responsive factor, Si-Defensin, Si-Chitinase, and Si-Thaumatin-like protein were monitored. The transgenic lines showed greater survival under different stresses compared to control through the integrated activation of multiple components of the defense signaling cascade. This is the first report of transgenic sesame and first of any study done on defense-related genes in sesame. This is also the first attempt at understanding the molecular mechanism underlying multi-stress tolerance imparted by an OLP.
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spelling pubmed-53682222017-04-11 Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame Chowdhury, Supriyo Basu, Arpita Kundu, Surekha Front Plant Sci Plant Science Osmotin-like proteins (OLPs), of PR-5 family, mediate defense against abiotic, and biotic stresses in plants. Overexpression in sesame of an OLP gene (SindOLP), enhanced tolerance against drought, salinity, oxidative stress, and the charcoal rot pathogen. SindOLP was expressed in all parts and localized to the cytosol. The transgenic plants recovered after prolonged drought and salinity stress, showing less electrolyte leakage, more water content, longer roots, and smaller stomatal aperture compared to control plants. There was an increase in osmolytes, ROS-scavenging enzymes, chlorophyll content, proline, secondary metabolites, and reduced lipid peroxidation in the transgenic sesame under multiple stresses. The OLP gene imparted increased tolerance through the increased expression of three genes coding for ROS scavenging enzymes and five defense-related marker genes functioning in the JA/ET and SA pathways, namely Si-Apetala2, Si-Ethylene-responsive factor, Si-Defensin, Si-Chitinase, and Si-Thaumatin-like protein were monitored. The transgenic lines showed greater survival under different stresses compared to control through the integrated activation of multiple components of the defense signaling cascade. This is the first report of transgenic sesame and first of any study done on defense-related genes in sesame. This is also the first attempt at understanding the molecular mechanism underlying multi-stress tolerance imparted by an OLP. Frontiers Media S.A. 2017-03-28 /pmc/articles/PMC5368222/ /pubmed/28400780 http://dx.doi.org/10.3389/fpls.2017.00410 Text en Copyright © 2017 Chowdhury, Basu and Kundu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chowdhury, Supriyo
Basu, Arpita
Kundu, Surekha
Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame
title Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame
title_full Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame
title_fullStr Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame
title_full_unstemmed Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame
title_short Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame
title_sort overexpression of a new osmotin-like protein gene (sindolp) confers tolerance against biotic and abiotic stresses in sesame
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368222/
https://www.ncbi.nlm.nih.gov/pubmed/28400780
http://dx.doi.org/10.3389/fpls.2017.00410
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