Cargando…
Ferulic acid promoting apoptosis in human osteosarcoma cell lines
OBJECTIVE: To explore the promoting apoptosis and antitumor activities of ferulic acid (FA) in human osteosarcoma and its potential mechanism. METHODS: The SaOS-2 and MG63 osteosarcoma cell lines were opted to experiment and these cells were, respectively, cultured with various concentrations of FA...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Professional Medical Publications
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368292/ https://www.ncbi.nlm.nih.gov/pubmed/28367185 http://dx.doi.org/10.12669/pjms.331.12066 |
_version_ | 1782517900629770240 |
---|---|
author | Zhang, Xu-dong Wu, Qiang Yang, Shu-hua |
author_facet | Zhang, Xu-dong Wu, Qiang Yang, Shu-hua |
author_sort | Zhang, Xu-dong |
collection | PubMed |
description | OBJECTIVE: To explore the promoting apoptosis and antitumor activities of ferulic acid (FA) in human osteosarcoma and its potential mechanism. METHODS: The SaOS-2 and MG63 osteosarcoma cell lines were opted to experiment and these cells were, respectively, cultured with various concentrations of FA (0 μM, 10 μM, 20 μM, 40 μM) for 72 hours at 37°C. The viabilities of the FA treated cells were monitored by MTT. Apoptosis cells were evaluated using annexin V/PI by flow cytometry. Apoptosis proteins caspase-3, procaspase-3, Bcl-2 and Bax were detected by western blot. Expressions of apoptotic genes Bcl-2 and Bax were quantified by qPCR. RESULTS: The cell viabilities were critically declined in the concentration-dependent manner in FA groups (P < 0.01). The apoptosis cells were increased proportionately with the concentration of FA (P < 0.05). The procaspase-3 protein contents, and Bcl-2 mRNA and protein contents were significantly decreased while caspase-3 protein contents, and Bax mRNA and protein contents were concomitantly increased in the concentration-dependent manner in FA groups (P < 0.05). The response to FA by the SaOS-2 osteosarcoma cell was similar with the MG63 osteosarcoma cell (P > 0.05). CONCLUSION: Ferulic acid could significantly descend osteosarcoma cell viability through the promoting apoptosis pathway in which FA activates both caspase-3 and Bax and inactivates Bcl-2. |
format | Online Article Text |
id | pubmed-5368292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Professional Medical Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-53682922017-03-31 Ferulic acid promoting apoptosis in human osteosarcoma cell lines Zhang, Xu-dong Wu, Qiang Yang, Shu-hua Pak J Med Sci Original Article OBJECTIVE: To explore the promoting apoptosis and antitumor activities of ferulic acid (FA) in human osteosarcoma and its potential mechanism. METHODS: The SaOS-2 and MG63 osteosarcoma cell lines were opted to experiment and these cells were, respectively, cultured with various concentrations of FA (0 μM, 10 μM, 20 μM, 40 μM) for 72 hours at 37°C. The viabilities of the FA treated cells were monitored by MTT. Apoptosis cells were evaluated using annexin V/PI by flow cytometry. Apoptosis proteins caspase-3, procaspase-3, Bcl-2 and Bax were detected by western blot. Expressions of apoptotic genes Bcl-2 and Bax were quantified by qPCR. RESULTS: The cell viabilities were critically declined in the concentration-dependent manner in FA groups (P < 0.01). The apoptosis cells were increased proportionately with the concentration of FA (P < 0.05). The procaspase-3 protein contents, and Bcl-2 mRNA and protein contents were significantly decreased while caspase-3 protein contents, and Bax mRNA and protein contents were concomitantly increased in the concentration-dependent manner in FA groups (P < 0.05). The response to FA by the SaOS-2 osteosarcoma cell was similar with the MG63 osteosarcoma cell (P > 0.05). CONCLUSION: Ferulic acid could significantly descend osteosarcoma cell viability through the promoting apoptosis pathway in which FA activates both caspase-3 and Bax and inactivates Bcl-2. Professional Medical Publications 2017 /pmc/articles/PMC5368292/ /pubmed/28367185 http://dx.doi.org/10.12669/pjms.331.12066 Text en Copyright: © Pakistan Journal of Medical Sciences http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zhang, Xu-dong Wu, Qiang Yang, Shu-hua Ferulic acid promoting apoptosis in human osteosarcoma cell lines |
title | Ferulic acid promoting apoptosis in human osteosarcoma cell lines |
title_full | Ferulic acid promoting apoptosis in human osteosarcoma cell lines |
title_fullStr | Ferulic acid promoting apoptosis in human osteosarcoma cell lines |
title_full_unstemmed | Ferulic acid promoting apoptosis in human osteosarcoma cell lines |
title_short | Ferulic acid promoting apoptosis in human osteosarcoma cell lines |
title_sort | ferulic acid promoting apoptosis in human osteosarcoma cell lines |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368292/ https://www.ncbi.nlm.nih.gov/pubmed/28367185 http://dx.doi.org/10.12669/pjms.331.12066 |
work_keys_str_mv | AT zhangxudong ferulicacidpromotingapoptosisinhumanosteosarcomacelllines AT wuqiang ferulicacidpromotingapoptosisinhumanosteosarcomacelllines AT yangshuhua ferulicacidpromotingapoptosisinhumanosteosarcomacelllines |