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Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana

Eucalyptus species are cultivated for forestry and are of economic importance. The fungal stem canker pathogen Chrysoporthe austroafricana causes disease of varying severity on E. grandis. The Eucalyptus grandis-Chrysoporthe austroafricana interaction has been established as a model system for study...

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Autores principales: Zwart, Lizahn, Berger, Dave Kenneth, Moleleki, Lucy Novungayo, van der Merwe, Nicolaas A., Myburg, Alexander A., Naidoo, Sanushka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368643/
https://www.ncbi.nlm.nih.gov/pubmed/28349984
http://dx.doi.org/10.1038/srep45402
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author Zwart, Lizahn
Berger, Dave Kenneth
Moleleki, Lucy Novungayo
van der Merwe, Nicolaas A.
Myburg, Alexander A.
Naidoo, Sanushka
author_facet Zwart, Lizahn
Berger, Dave Kenneth
Moleleki, Lucy Novungayo
van der Merwe, Nicolaas A.
Myburg, Alexander A.
Naidoo, Sanushka
author_sort Zwart, Lizahn
collection PubMed
description Eucalyptus species are cultivated for forestry and are of economic importance. The fungal stem canker pathogen Chrysoporthe austroafricana causes disease of varying severity on E. grandis. The Eucalyptus grandis-Chrysoporthe austroafricana interaction has been established as a model system for studying Eucalyptus antifungal defence. Previous studies revealed that the phytohormone salicylic acid (SA) affects the levels of resistance in highly susceptible (ZG14) and moderately resistant (TAG5) clones. The aims of this study were to examine histochemical changes in response to wounding and inoculation as well as host responses at the protein level. The anatomy and histochemical changes induced by wounding and inoculation were similar between the clones, suggesting that anatomical differences do not underlie their different levels of resistance. Tyloses and gum-like substances were present after inoculation and wounding, but cell death occurred only after inoculation. Hyphae of C. austroafricana were observed inside dead and living cells, suggesting that the possibility of a hemibiotrophic interaction requires further investigation. Proteomics analysis revealed the possible involvement of proteins associated with cell death, SA signalling and systemic resistance. In combination with previous information, this study forms a basis for future functional characterisation of candidate genes involved in resistance of E. grandis to C. austroafricana.
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spelling pubmed-53686432017-03-30 Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana Zwart, Lizahn Berger, Dave Kenneth Moleleki, Lucy Novungayo van der Merwe, Nicolaas A. Myburg, Alexander A. Naidoo, Sanushka Sci Rep Article Eucalyptus species are cultivated for forestry and are of economic importance. The fungal stem canker pathogen Chrysoporthe austroafricana causes disease of varying severity on E. grandis. The Eucalyptus grandis-Chrysoporthe austroafricana interaction has been established as a model system for studying Eucalyptus antifungal defence. Previous studies revealed that the phytohormone salicylic acid (SA) affects the levels of resistance in highly susceptible (ZG14) and moderately resistant (TAG5) clones. The aims of this study were to examine histochemical changes in response to wounding and inoculation as well as host responses at the protein level. The anatomy and histochemical changes induced by wounding and inoculation were similar between the clones, suggesting that anatomical differences do not underlie their different levels of resistance. Tyloses and gum-like substances were present after inoculation and wounding, but cell death occurred only after inoculation. Hyphae of C. austroafricana were observed inside dead and living cells, suggesting that the possibility of a hemibiotrophic interaction requires further investigation. Proteomics analysis revealed the possible involvement of proteins associated with cell death, SA signalling and systemic resistance. In combination with previous information, this study forms a basis for future functional characterisation of candidate genes involved in resistance of E. grandis to C. austroafricana. Nature Publishing Group 2017-03-28 /pmc/articles/PMC5368643/ /pubmed/28349984 http://dx.doi.org/10.1038/srep45402 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zwart, Lizahn
Berger, Dave Kenneth
Moleleki, Lucy Novungayo
van der Merwe, Nicolaas A.
Myburg, Alexander A.
Naidoo, Sanushka
Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana
title Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana
title_full Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana
title_fullStr Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana
title_full_unstemmed Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana
title_short Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana
title_sort evidence for salicylic acid signalling and histological changes in the defence response of eucalyptus grandis to chrysoporthe austroafricana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5368643/
https://www.ncbi.nlm.nih.gov/pubmed/28349984
http://dx.doi.org/10.1038/srep45402
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