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Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
Development of nanoparticles for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical probes for biological studies. Moreover, sriNPs enable us to monitor the behavior of engineered nanomaterials in complex biological environments with high spatial resolution, which is...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369336/ https://www.ncbi.nlm.nih.gov/pubmed/28451345 http://dx.doi.org/10.1039/c6sc04664a |
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author | Shang, Li Gao, Peng Wang, Haixia Popescu, Radian Gerthsen, Dagmar Nienhaus, Gerd Ulrich |
author_facet | Shang, Li Gao, Peng Wang, Haixia Popescu, Radian Gerthsen, Dagmar Nienhaus, Gerd Ulrich |
author_sort | Shang, Li |
collection | PubMed |
description | Development of nanoparticles for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical probes for biological studies. Moreover, sriNPs enable us to monitor the behavior of engineered nanomaterials in complex biological environments with high spatial resolution, which is important for advancing our understanding of nano–bio interactions. Up to now, reports on sriNPs have been scarce. In this work, we report a facile strategy to prepare protein-based fluorescent NPs that can be utilized as probes in super-resolution microscopy. The method is simple and straightforward, and easily extendible to other types of fluorophores. By using Atto647N–transferrin NPs as an example, we have achieved a roughly four-fold resolution improvement by using STED nanoscopy. These protein-based sriNPs possess excellent biocompatibility, good colloidal stability and photostability, making them attractive candidates for biological studies. Moreover, STED nanoscopy enables the precise imaging of NP structures in living cells, and revealed the co-existence of multiple NPs within one endosomal vesicle. |
format | Online Article Text |
id | pubmed-5369336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-53693362017-04-27 Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells Shang, Li Gao, Peng Wang, Haixia Popescu, Radian Gerthsen, Dagmar Nienhaus, Gerd Ulrich Chem Sci Chemistry Development of nanoparticles for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical probes for biological studies. Moreover, sriNPs enable us to monitor the behavior of engineered nanomaterials in complex biological environments with high spatial resolution, which is important for advancing our understanding of nano–bio interactions. Up to now, reports on sriNPs have been scarce. In this work, we report a facile strategy to prepare protein-based fluorescent NPs that can be utilized as probes in super-resolution microscopy. The method is simple and straightforward, and easily extendible to other types of fluorophores. By using Atto647N–transferrin NPs as an example, we have achieved a roughly four-fold resolution improvement by using STED nanoscopy. These protein-based sriNPs possess excellent biocompatibility, good colloidal stability and photostability, making them attractive candidates for biological studies. Moreover, STED nanoscopy enables the precise imaging of NP structures in living cells, and revealed the co-existence of multiple NPs within one endosomal vesicle. Royal Society of Chemistry 2017-03-01 2016-12-19 /pmc/articles/PMC5369336/ /pubmed/28451345 http://dx.doi.org/10.1039/c6sc04664a Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Shang, Li Gao, Peng Wang, Haixia Popescu, Radian Gerthsen, Dagmar Nienhaus, Gerd Ulrich Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells |
title | Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
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title_full | Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
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title_fullStr | Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
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title_full_unstemmed | Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
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title_short | Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
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title_sort | protein-based fluorescent nanoparticles for super-resolution sted imaging of live cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369336/ https://www.ncbi.nlm.nih.gov/pubmed/28451345 http://dx.doi.org/10.1039/c6sc04664a |
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