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Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells

Development of nanoparticles for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical probes for biological studies. Moreover, sriNPs enable us to monitor the behavior of engineered nanomaterials in complex biological environments with high spatial resolution, which is...

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Detalles Bibliográficos
Autores principales: Shang, Li, Gao, Peng, Wang, Haixia, Popescu, Radian, Gerthsen, Dagmar, Nienhaus, Gerd Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369336/
https://www.ncbi.nlm.nih.gov/pubmed/28451345
http://dx.doi.org/10.1039/c6sc04664a
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author Shang, Li
Gao, Peng
Wang, Haixia
Popescu, Radian
Gerthsen, Dagmar
Nienhaus, Gerd Ulrich
author_facet Shang, Li
Gao, Peng
Wang, Haixia
Popescu, Radian
Gerthsen, Dagmar
Nienhaus, Gerd Ulrich
author_sort Shang, Li
collection PubMed
description Development of nanoparticles for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical probes for biological studies. Moreover, sriNPs enable us to monitor the behavior of engineered nanomaterials in complex biological environments with high spatial resolution, which is important for advancing our understanding of nano–bio interactions. Up to now, reports on sriNPs have been scarce. In this work, we report a facile strategy to prepare protein-based fluorescent NPs that can be utilized as probes in super-resolution microscopy. The method is simple and straightforward, and easily extendible to other types of fluorophores. By using Atto647N–transferrin NPs as an example, we have achieved a roughly four-fold resolution improvement by using STED nanoscopy. These protein-based sriNPs possess excellent biocompatibility, good colloidal stability and photostability, making them attractive candidates for biological studies. Moreover, STED nanoscopy enables the precise imaging of NP structures in living cells, and revealed the co-existence of multiple NPs within one endosomal vesicle.
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spelling pubmed-53693362017-04-27 Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells Shang, Li Gao, Peng Wang, Haixia Popescu, Radian Gerthsen, Dagmar Nienhaus, Gerd Ulrich Chem Sci Chemistry Development of nanoparticles for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical probes for biological studies. Moreover, sriNPs enable us to monitor the behavior of engineered nanomaterials in complex biological environments with high spatial resolution, which is important for advancing our understanding of nano–bio interactions. Up to now, reports on sriNPs have been scarce. In this work, we report a facile strategy to prepare protein-based fluorescent NPs that can be utilized as probes in super-resolution microscopy. The method is simple and straightforward, and easily extendible to other types of fluorophores. By using Atto647N–transferrin NPs as an example, we have achieved a roughly four-fold resolution improvement by using STED nanoscopy. These protein-based sriNPs possess excellent biocompatibility, good colloidal stability and photostability, making them attractive candidates for biological studies. Moreover, STED nanoscopy enables the precise imaging of NP structures in living cells, and revealed the co-existence of multiple NPs within one endosomal vesicle. Royal Society of Chemistry 2017-03-01 2016-12-19 /pmc/articles/PMC5369336/ /pubmed/28451345 http://dx.doi.org/10.1039/c6sc04664a Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Shang, Li
Gao, Peng
Wang, Haixia
Popescu, Radian
Gerthsen, Dagmar
Nienhaus, Gerd Ulrich
Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
title Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
title_full Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
title_fullStr Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
title_full_unstemmed Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
title_short Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells
title_sort protein-based fluorescent nanoparticles for super-resolution sted imaging of live cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369336/
https://www.ncbi.nlm.nih.gov/pubmed/28451345
http://dx.doi.org/10.1039/c6sc04664a
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