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Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase

Sertoli cells are required for normal spermatogenesis and they can be reprogrammed to other types of functional cells. However, the number of primary Sertoli cells is rare and human Sertoli cell line is unavailable. In this study, we have for the first time reported a stable human Sertoli cell line,...

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Autores principales: Wen, Liping, Yuan, Qingqing, Sun, Min, Niu, Minghui, Wang, Hong, Fu, Hongyong, Zhou, Fan, Yao, Chencheng, Wang, Xiaobo, Li, Zheng, He, Zuping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369984/
https://www.ncbi.nlm.nih.gov/pubmed/28152522
http://dx.doi.org/10.18632/oncotarget.14985
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author Wen, Liping
Yuan, Qingqing
Sun, Min
Niu, Minghui
Wang, Hong
Fu, Hongyong
Zhou, Fan
Yao, Chencheng
Wang, Xiaobo
Li, Zheng
He, Zuping
author_facet Wen, Liping
Yuan, Qingqing
Sun, Min
Niu, Minghui
Wang, Hong
Fu, Hongyong
Zhou, Fan
Yao, Chencheng
Wang, Xiaobo
Li, Zheng
He, Zuping
author_sort Wen, Liping
collection PubMed
description Sertoli cells are required for normal spermatogenesis and they can be reprogrammed to other types of functional cells. However, the number of primary Sertoli cells is rare and human Sertoli cell line is unavailable. In this study, we have for the first time reported a stable human Sertoli cell line, namely hS1 cells, by overexpression of human telomerase. The hS1 cells expressed a number of hallmarks for human Sertoli cells, including SOX9, WT1, GDNF, SCF, BMP4, BMP6, GATA4, and VIM, and they were negative for 3β-HSD, SMA, and VASA. Higher levels of AR and FSHR were observed in hS1 cells compared to primary human Sertoli cells. Microarray analysis showed that 70.4% of global gene profiles of hS1 cells were similar to primary human Sertoli cells. Proliferation assay demonstrated that hS1 cells proliferated rapidly and they could be passaged for more than 30 times in 6 months. Neither Y chromosome microdeletion nor tumorgenesis was detected in this cell line and 90% normal karyotypes existed in hS1 cells. Collectively, we have established the first human Sertoli cell line with phenotype of primary human Sertoli cells, an unlimited proliferation potential and high safety, which could offer sufficient human Sertoli cells for basic research as well as reproductive and regenerative medicine.
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spelling pubmed-53699842017-04-17 Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase Wen, Liping Yuan, Qingqing Sun, Min Niu, Minghui Wang, Hong Fu, Hongyong Zhou, Fan Yao, Chencheng Wang, Xiaobo Li, Zheng He, Zuping Oncotarget Research Paper Sertoli cells are required for normal spermatogenesis and they can be reprogrammed to other types of functional cells. However, the number of primary Sertoli cells is rare and human Sertoli cell line is unavailable. In this study, we have for the first time reported a stable human Sertoli cell line, namely hS1 cells, by overexpression of human telomerase. The hS1 cells expressed a number of hallmarks for human Sertoli cells, including SOX9, WT1, GDNF, SCF, BMP4, BMP6, GATA4, and VIM, and they were negative for 3β-HSD, SMA, and VASA. Higher levels of AR and FSHR were observed in hS1 cells compared to primary human Sertoli cells. Microarray analysis showed that 70.4% of global gene profiles of hS1 cells were similar to primary human Sertoli cells. Proliferation assay demonstrated that hS1 cells proliferated rapidly and they could be passaged for more than 30 times in 6 months. Neither Y chromosome microdeletion nor tumorgenesis was detected in this cell line and 90% normal karyotypes existed in hS1 cells. Collectively, we have established the first human Sertoli cell line with phenotype of primary human Sertoli cells, an unlimited proliferation potential and high safety, which could offer sufficient human Sertoli cells for basic research as well as reproductive and regenerative medicine. Impact Journals LLC 2017-01-27 /pmc/articles/PMC5369984/ /pubmed/28152522 http://dx.doi.org/10.18632/oncotarget.14985 Text en Copyright: © 2017 Wen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wen, Liping
Yuan, Qingqing
Sun, Min
Niu, Minghui
Wang, Hong
Fu, Hongyong
Zhou, Fan
Yao, Chencheng
Wang, Xiaobo
Li, Zheng
He, Zuping
Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase
title Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase
title_full Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase
title_fullStr Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase
title_full_unstemmed Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase
title_short Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase
title_sort generation and characteristics of human sertoli cell line immortalized by overexpression of human telomerase
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369984/
https://www.ncbi.nlm.nih.gov/pubmed/28152522
http://dx.doi.org/10.18632/oncotarget.14985
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