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Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA

Using the phoA-fusion technology, the recombinant metallothionein (MT) from freshwater crab (Sinopotamon yangtsekiense) has been successfully produced in Escherichia coli. MT purified from the bacterial suspension showed one polypeptide with a molecular weight of 7 kDa by tricine-sodium dodecyl sulf...

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Detalles Bibliográficos
Autores principales: Yang, Jian, Sun, Hui, Zhang, Hao, Zhou, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370132/
https://www.ncbi.nlm.nih.gov/pubmed/28350826
http://dx.doi.org/10.1371/journal.pone.0174482

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