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Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures
Vesivirus 2117 is an adventitious agent that, in 2009, was identified as a contaminant of Chinese hamster ovary cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme. The consequent interruption in supply of Fabrazyme and Cerezyme (drugs used to treat Fabry and...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370393/ https://www.ncbi.nlm.nih.gov/pubmed/27902397 http://dx.doi.org/10.1099/jgv.0.000658 |
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author | Conley, Michaela Emmott, Edward Orton, Richard Taylor, David Carneiro, Daniel G Murata, Kazuyoshi Goodfellow, Ian G Hansman, Grant S Bhella, David |
author_facet | Conley, Michaela Emmott, Edward Orton, Richard Taylor, David Carneiro, Daniel G Murata, Kazuyoshi Goodfellow, Ian G Hansman, Grant S Bhella, David |
author_sort | Conley, Michaela |
collection | PubMed |
description | Vesivirus 2117 is an adventitious agent that, in 2009, was identified as a contaminant of Chinese hamster ovary cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme. The consequent interruption in supply of Fabrazyme and Cerezyme (drugs used to treat Fabry and Gaucher diseases, respectively) caused significant economic losses. Vesivirus 2117 is a member of the Caliciviridae, a family of small icosahedral viruses encoding a positive-sense RNA genome. We have used cryo-electron microscopy and three-dimensional image reconstruction to calculate a structure of vesivirus 2117 virus-like particles as well as feline calicivirus and a chimeric sapovirus. We present a structural comparison of several members of the Caliciviridae, showing that the distal P domain of vesivirus 2117 is morphologically distinct from that seen in other known vesivirus structures. Furthermore, at intermediate resolutions, we found a high level of structural similarity between vesivirus 2117 and Caliciviridae from other genera: sapovirus and rabbit hemorrhagic disease virus. Phylogenetic analysis confirms vesivirus 2117 as a vesivirus closely related to canine vesiviruses. We postulate that morphological differences in virion structure seen between vesivirus clades may reflect differences in receptor usage. |
format | Online Article Text |
id | pubmed-5370393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-53703932017-05-02 Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures Conley, Michaela Emmott, Edward Orton, Richard Taylor, David Carneiro, Daniel G Murata, Kazuyoshi Goodfellow, Ian G Hansman, Grant S Bhella, David J Gen Virol Research Article Vesivirus 2117 is an adventitious agent that, in 2009, was identified as a contaminant of Chinese hamster ovary cells propagated in bioreactors at a pharmaceutical manufacturing plant belonging to Genzyme. The consequent interruption in supply of Fabrazyme and Cerezyme (drugs used to treat Fabry and Gaucher diseases, respectively) caused significant economic losses. Vesivirus 2117 is a member of the Caliciviridae, a family of small icosahedral viruses encoding a positive-sense RNA genome. We have used cryo-electron microscopy and three-dimensional image reconstruction to calculate a structure of vesivirus 2117 virus-like particles as well as feline calicivirus and a chimeric sapovirus. We present a structural comparison of several members of the Caliciviridae, showing that the distal P domain of vesivirus 2117 is morphologically distinct from that seen in other known vesivirus structures. Furthermore, at intermediate resolutions, we found a high level of structural similarity between vesivirus 2117 and Caliciviridae from other genera: sapovirus and rabbit hemorrhagic disease virus. Phylogenetic analysis confirms vesivirus 2117 as a vesivirus closely related to canine vesiviruses. We postulate that morphological differences in virion structure seen between vesivirus clades may reflect differences in receptor usage. Microbiology Society 2017-01 2017-03-17 /pmc/articles/PMC5370393/ /pubmed/27902397 http://dx.doi.org/10.1099/jgv.0.000658 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Conley, Michaela Emmott, Edward Orton, Richard Taylor, David Carneiro, Daniel G Murata, Kazuyoshi Goodfellow, Ian G Hansman, Grant S Bhella, David Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures |
title | Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures |
title_full | Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures |
title_fullStr | Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures |
title_full_unstemmed | Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures |
title_short | Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures |
title_sort | vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370393/ https://www.ncbi.nlm.nih.gov/pubmed/27902397 http://dx.doi.org/10.1099/jgv.0.000658 |
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