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Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study

An essential question in studies on the origins of life is how nucleic acids were first synthesized and then incorporated into compartments about 4 billion years ago. A recent discovery is that guided polymerization within organizing matrices could promote a non-enzymatic condensation reaction allow...

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Autores principales: Misuraca, Loreto, Natali, Francesca, da Silva, Laura, Peters, Judith, Demé, Bruno, Ollivier, Jacques, Seydel, Tilo, Laux-Lesourd, Valerie, Haertlein, Michael, Zaccai, Giuseppe, Deamer, David, Maurel, Marie Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370402/
https://www.ncbi.nlm.nih.gov/pubmed/28054992
http://dx.doi.org/10.3390/life7010002
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author Misuraca, Loreto
Natali, Francesca
da Silva, Laura
Peters, Judith
Demé, Bruno
Ollivier, Jacques
Seydel, Tilo
Laux-Lesourd, Valerie
Haertlein, Michael
Zaccai, Giuseppe
Deamer, David
Maurel, Marie Christine
author_facet Misuraca, Loreto
Natali, Francesca
da Silva, Laura
Peters, Judith
Demé, Bruno
Ollivier, Jacques
Seydel, Tilo
Laux-Lesourd, Valerie
Haertlein, Michael
Zaccai, Giuseppe
Deamer, David
Maurel, Marie Christine
author_sort Misuraca, Loreto
collection PubMed
description An essential question in studies on the origins of life is how nucleic acids were first synthesized and then incorporated into compartments about 4 billion years ago. A recent discovery is that guided polymerization within organizing matrices could promote a non-enzymatic condensation reaction allowing the formation of RNA-like polymers, followed by encapsulation in lipid membranes. Here, we used neutron scattering and deuterium labelling to investigate 5′-adenosine monophosphate (AMP) molecules captured in a multilamellar phospholipid matrix. The aim of the research was to determine and compare how mononucleotides are captured and differently organized within matrices and multilamellar phospholipid structures and to explore the role of water in organizing the system to determine at which level the system becomes sufficiently anhydrous to lock the AMP molecules into an organized structure and initiate ester bond synthesis. Elastic incoherent neutron scattering experiments were thus employed to investigate the changes of the dynamic properties of AMP induced by embedding the molecules within the lipid matrix. The influence of AMP addition to the lipid membrane organization was determined through diffraction measurement, which also helped us to define the best working Q range for dynamical data analysis with respect to specific hydration. The use of different complementary instruments allowed coverage of a wide time-scale domain, from ns to ps, of atomic mean square fluctuations, providing evidence of a well-defined dependence of the AMP dynamics on the hydration level.
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spelling pubmed-53704022017-04-05 Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study Misuraca, Loreto Natali, Francesca da Silva, Laura Peters, Judith Demé, Bruno Ollivier, Jacques Seydel, Tilo Laux-Lesourd, Valerie Haertlein, Michael Zaccai, Giuseppe Deamer, David Maurel, Marie Christine Life (Basel) Article An essential question in studies on the origins of life is how nucleic acids were first synthesized and then incorporated into compartments about 4 billion years ago. A recent discovery is that guided polymerization within organizing matrices could promote a non-enzymatic condensation reaction allowing the formation of RNA-like polymers, followed by encapsulation in lipid membranes. Here, we used neutron scattering and deuterium labelling to investigate 5′-adenosine monophosphate (AMP) molecules captured in a multilamellar phospholipid matrix. The aim of the research was to determine and compare how mononucleotides are captured and differently organized within matrices and multilamellar phospholipid structures and to explore the role of water in organizing the system to determine at which level the system becomes sufficiently anhydrous to lock the AMP molecules into an organized structure and initiate ester bond synthesis. Elastic incoherent neutron scattering experiments were thus employed to investigate the changes of the dynamic properties of AMP induced by embedding the molecules within the lipid matrix. The influence of AMP addition to the lipid membrane organization was determined through diffraction measurement, which also helped us to define the best working Q range for dynamical data analysis with respect to specific hydration. The use of different complementary instruments allowed coverage of a wide time-scale domain, from ns to ps, of atomic mean square fluctuations, providing evidence of a well-defined dependence of the AMP dynamics on the hydration level. MDPI 2017-01-04 /pmc/articles/PMC5370402/ /pubmed/28054992 http://dx.doi.org/10.3390/life7010002 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Misuraca, Loreto
Natali, Francesca
da Silva, Laura
Peters, Judith
Demé, Bruno
Ollivier, Jacques
Seydel, Tilo
Laux-Lesourd, Valerie
Haertlein, Michael
Zaccai, Giuseppe
Deamer, David
Maurel, Marie Christine
Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study
title Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study
title_full Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study
title_fullStr Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study
title_full_unstemmed Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study
title_short Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study
title_sort mobility of a mononucleotide within a lipid matrix: a neutron scattering study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370402/
https://www.ncbi.nlm.nih.gov/pubmed/28054992
http://dx.doi.org/10.3390/life7010002
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