Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts

Keloid is a common and refractory disease characterized by abnormal fibroblast proliferation and excessive deposition of extracellular matrix components. Hypocrellin B (HB) is a natural perylene quinone photosensitizer. In this experiment, we studied the effects of photodynamic therapy (PDT) using y...

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Autores principales: Hu, Yongqing, Zhang, Chunmin, Li, Shengli, Jiao, Ya, Qi, Tonggang, Wei, Guo, Han, Gangwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370439/
https://www.ncbi.nlm.nih.gov/pubmed/28367096
http://dx.doi.org/10.7150/ijbs.17920
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author Hu, Yongqing
Zhang, Chunmin
Li, Shengli
Jiao, Ya
Qi, Tonggang
Wei, Guo
Han, Gangwen
author_facet Hu, Yongqing
Zhang, Chunmin
Li, Shengli
Jiao, Ya
Qi, Tonggang
Wei, Guo
Han, Gangwen
author_sort Hu, Yongqing
collection PubMed
description Keloid is a common and refractory disease characterized by abnormal fibroblast proliferation and excessive deposition of extracellular matrix components. Hypocrellin B (HB) is a natural perylene quinone photosensitizer. In this experiment, we studied the effects of photodynamic therapy (PDT) using yellow light from light-emitting diode (LED) combined with HB on keloid fibroblasts (KFB) in vitro. Our results showed that HB-LED PDT treatment induced significant KFB apoptosis and decreased KFB cell viability. HB-LED PDT treatment lead to significant BAX upregulation and BCL-2 downregulation in KFB cells, which led to elevation of intracellular free Ca(2+) and activation of caspase-3. Our data provides preliminary evidence for the potential of HB-LED PDT as a therapeutic strategy for keloid.
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spelling pubmed-53704392017-03-31 Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts Hu, Yongqing Zhang, Chunmin Li, Shengli Jiao, Ya Qi, Tonggang Wei, Guo Han, Gangwen Int J Biol Sci Research Paper Keloid is a common and refractory disease characterized by abnormal fibroblast proliferation and excessive deposition of extracellular matrix components. Hypocrellin B (HB) is a natural perylene quinone photosensitizer. In this experiment, we studied the effects of photodynamic therapy (PDT) using yellow light from light-emitting diode (LED) combined with HB on keloid fibroblasts (KFB) in vitro. Our results showed that HB-LED PDT treatment induced significant KFB apoptosis and decreased KFB cell viability. HB-LED PDT treatment lead to significant BAX upregulation and BCL-2 downregulation in KFB cells, which led to elevation of intracellular free Ca(2+) and activation of caspase-3. Our data provides preliminary evidence for the potential of HB-LED PDT as a therapeutic strategy for keloid. Ivyspring International Publisher 2017-02-25 /pmc/articles/PMC5370439/ /pubmed/28367096 http://dx.doi.org/10.7150/ijbs.17920 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Hu, Yongqing
Zhang, Chunmin
Li, Shengli
Jiao, Ya
Qi, Tonggang
Wei, Guo
Han, Gangwen
Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
title Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
title_full Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
title_fullStr Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
title_full_unstemmed Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
title_short Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
title_sort effects of photodynamic therapy using yellow led-light with concomitant hypocrellin b on apoptotic signaling in keloid fibroblasts
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370439/
https://www.ncbi.nlm.nih.gov/pubmed/28367096
http://dx.doi.org/10.7150/ijbs.17920
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