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Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells

Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (−)­epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In th...

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Autores principales: Yoshioka, Yasukiyo, Li, Xiu, Zhang, Tianshun, Mitani, Takakazu, Yasuda, Michiko, Nanba, Fumio, Toda, Toshiya, Yamashita, Yoko, Ashida, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370529/
https://www.ncbi.nlm.nih.gov/pubmed/28366989
http://dx.doi.org/10.3164/jcbn.16-48
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author Yoshioka, Yasukiyo
Li, Xiu
Zhang, Tianshun
Mitani, Takakazu
Yasuda, Michiko
Nanba, Fumio
Toda, Toshiya
Yamashita, Yoko
Ashida, Hitoshi
author_facet Yoshioka, Yasukiyo
Li, Xiu
Zhang, Tianshun
Mitani, Takakazu
Yasuda, Michiko
Nanba, Fumio
Toda, Toshiya
Yamashita, Yoko
Ashida, Hitoshi
author_sort Yoshioka, Yasukiyo
collection PubMed
description Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (−)­epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages.
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spelling pubmed-53705292017-03-31 Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells Yoshioka, Yasukiyo Li, Xiu Zhang, Tianshun Mitani, Takakazu Yasuda, Michiko Nanba, Fumio Toda, Toshiya Yamashita, Yoko Ashida, Hitoshi J Clin Biochem Nutr Original Article Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (−)­epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages. the Society for Free Radical Research Japan 2017-03 2016-12-06 /pmc/articles/PMC5370529/ /pubmed/28366989 http://dx.doi.org/10.3164/jcbn.16-48 Text en Copyright © 2017 JCBN http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yoshioka, Yasukiyo
Li, Xiu
Zhang, Tianshun
Mitani, Takakazu
Yasuda, Michiko
Nanba, Fumio
Toda, Toshiya
Yamashita, Yoko
Ashida, Hitoshi
Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells
title Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells
title_full Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells
title_fullStr Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells
title_full_unstemmed Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells
title_short Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells
title_sort black soybean seed coat polyphenols prevent aaph-induced oxidative dna-damage in hepg2 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370529/
https://www.ncbi.nlm.nih.gov/pubmed/28366989
http://dx.doi.org/10.3164/jcbn.16-48
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