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Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells
Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (−)epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Society for Free Radical Research Japan
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370529/ https://www.ncbi.nlm.nih.gov/pubmed/28366989 http://dx.doi.org/10.3164/jcbn.16-48 |
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author | Yoshioka, Yasukiyo Li, Xiu Zhang, Tianshun Mitani, Takakazu Yasuda, Michiko Nanba, Fumio Toda, Toshiya Yamashita, Yoko Ashida, Hitoshi |
author_facet | Yoshioka, Yasukiyo Li, Xiu Zhang, Tianshun Mitani, Takakazu Yasuda, Michiko Nanba, Fumio Toda, Toshiya Yamashita, Yoko Ashida, Hitoshi |
author_sort | Yoshioka, Yasukiyo |
collection | PubMed |
description | Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (−)epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages. |
format | Online Article Text |
id | pubmed-5370529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-53705292017-03-31 Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells Yoshioka, Yasukiyo Li, Xiu Zhang, Tianshun Mitani, Takakazu Yasuda, Michiko Nanba, Fumio Toda, Toshiya Yamashita, Yoko Ashida, Hitoshi J Clin Biochem Nutr Original Article Black soybean seed coat extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (−)epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages. the Society for Free Radical Research Japan 2017-03 2016-12-06 /pmc/articles/PMC5370529/ /pubmed/28366989 http://dx.doi.org/10.3164/jcbn.16-48 Text en Copyright © 2017 JCBN http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yoshioka, Yasukiyo Li, Xiu Zhang, Tianshun Mitani, Takakazu Yasuda, Michiko Nanba, Fumio Toda, Toshiya Yamashita, Yoko Ashida, Hitoshi Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells |
title | Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells |
title_full | Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells |
title_fullStr | Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells |
title_full_unstemmed | Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells |
title_short | Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells |
title_sort | black soybean seed coat polyphenols prevent aaph-induced oxidative dna-damage in hepg2 cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5370529/ https://www.ncbi.nlm.nih.gov/pubmed/28366989 http://dx.doi.org/10.3164/jcbn.16-48 |
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