MiRNA expression in the cartilage of patients with osteoarthritis

BACKGROUND: Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. METHODS: Forty tissue samples from 29 patients undergoing joint rep...

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Autores principales: Kopańska, Marta, Szala, Dariusz, Czech, Joanna, Gabło, Natalia, Gargasz, Krzysztof, Trzeciak, Mateusz, Zawlik, Izabela, Snela, Sławomir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371266/
https://www.ncbi.nlm.nih.gov/pubmed/28351380
http://dx.doi.org/10.1186/s13018-017-0542-y
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author Kopańska, Marta
Szala, Dariusz
Czech, Joanna
Gabło, Natalia
Gargasz, Krzysztof
Trzeciak, Mateusz
Zawlik, Izabela
Snela, Sławomir
author_facet Kopańska, Marta
Szala, Dariusz
Czech, Joanna
Gabło, Natalia
Gargasz, Krzysztof
Trzeciak, Mateusz
Zawlik, Izabela
Snela, Sławomir
author_sort Kopańska, Marta
collection PubMed
description BACKGROUND: Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. METHODS: Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction. RESULTS: Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group). CONCLUSIONS: These findings may contribute to disease prevention and the development of therapeutic targets for OA.
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spelling pubmed-53712662017-03-30 MiRNA expression in the cartilage of patients with osteoarthritis Kopańska, Marta Szala, Dariusz Czech, Joanna Gabło, Natalia Gargasz, Krzysztof Trzeciak, Mateusz Zawlik, Izabela Snela, Sławomir J Orthop Surg Res Research Article BACKGROUND: Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. METHODS: Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction. RESULTS: Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group). CONCLUSIONS: These findings may contribute to disease prevention and the development of therapeutic targets for OA. BioMed Central 2017-03-28 /pmc/articles/PMC5371266/ /pubmed/28351380 http://dx.doi.org/10.1186/s13018-017-0542-y Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kopańska, Marta
Szala, Dariusz
Czech, Joanna
Gabło, Natalia
Gargasz, Krzysztof
Trzeciak, Mateusz
Zawlik, Izabela
Snela, Sławomir
MiRNA expression in the cartilage of patients with osteoarthritis
title MiRNA expression in the cartilage of patients with osteoarthritis
title_full MiRNA expression in the cartilage of patients with osteoarthritis
title_fullStr MiRNA expression in the cartilage of patients with osteoarthritis
title_full_unstemmed MiRNA expression in the cartilage of patients with osteoarthritis
title_short MiRNA expression in the cartilage of patients with osteoarthritis
title_sort mirna expression in the cartilage of patients with osteoarthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371266/
https://www.ncbi.nlm.nih.gov/pubmed/28351380
http://dx.doi.org/10.1186/s13018-017-0542-y
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