Cargando…
An alternative reference space for H&E color normalization
Digital imaging of H&E stained slides has enabled the application of image processing to support pathology workflows. Potential applications include computer-aided diagnostics, advanced quantification tools, and innovative visualization platforms. However, the intrinsic variability of biological...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371320/ https://www.ncbi.nlm.nih.gov/pubmed/28355298 http://dx.doi.org/10.1371/journal.pone.0174489 |
_version_ | 1782518399176278016 |
---|---|
author | Zarella, Mark D. Yeoh, Chan Breen, David E. Garcia, Fernando U. |
author_facet | Zarella, Mark D. Yeoh, Chan Breen, David E. Garcia, Fernando U. |
author_sort | Zarella, Mark D. |
collection | PubMed |
description | Digital imaging of H&E stained slides has enabled the application of image processing to support pathology workflows. Potential applications include computer-aided diagnostics, advanced quantification tools, and innovative visualization platforms. However, the intrinsic variability of biological tissue and the vast differences in tissue preparation protocols often lead to significant image variability that can hamper the effectiveness of these computational tools. We developed an alternative representation for H&E images that operates within a space that is more amenable to many of these image processing tools. The algorithm to derive this representation operates by exploiting the correlation between color and the spatial properties of the biological structures present in most H&E images. In this way, images are transformed into a structure-centric space in which images are segregated into tissue structure channels. We demonstrate that this framework can be extended to achieve color normalization, effectively reducing inter-slide variability. |
format | Online Article Text |
id | pubmed-5371320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53713202017-04-07 An alternative reference space for H&E color normalization Zarella, Mark D. Yeoh, Chan Breen, David E. Garcia, Fernando U. PLoS One Research Article Digital imaging of H&E stained slides has enabled the application of image processing to support pathology workflows. Potential applications include computer-aided diagnostics, advanced quantification tools, and innovative visualization platforms. However, the intrinsic variability of biological tissue and the vast differences in tissue preparation protocols often lead to significant image variability that can hamper the effectiveness of these computational tools. We developed an alternative representation for H&E images that operates within a space that is more amenable to many of these image processing tools. The algorithm to derive this representation operates by exploiting the correlation between color and the spatial properties of the biological structures present in most H&E images. In this way, images are transformed into a structure-centric space in which images are segregated into tissue structure channels. We demonstrate that this framework can be extended to achieve color normalization, effectively reducing inter-slide variability. Public Library of Science 2017-03-29 /pmc/articles/PMC5371320/ /pubmed/28355298 http://dx.doi.org/10.1371/journal.pone.0174489 Text en © 2017 Zarella et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zarella, Mark D. Yeoh, Chan Breen, David E. Garcia, Fernando U. An alternative reference space for H&E color normalization |
title | An alternative reference space for H&E color normalization |
title_full | An alternative reference space for H&E color normalization |
title_fullStr | An alternative reference space for H&E color normalization |
title_full_unstemmed | An alternative reference space for H&E color normalization |
title_short | An alternative reference space for H&E color normalization |
title_sort | alternative reference space for h&e color normalization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371320/ https://www.ncbi.nlm.nih.gov/pubmed/28355298 http://dx.doi.org/10.1371/journal.pone.0174489 |
work_keys_str_mv | AT zarellamarkd analternativereferencespaceforhecolornormalization AT yeohchan analternativereferencespaceforhecolornormalization AT breendavide analternativereferencespaceforhecolornormalization AT garciafernandou analternativereferencespaceforhecolornormalization AT zarellamarkd alternativereferencespaceforhecolornormalization AT yeohchan alternativereferencespaceforhecolornormalization AT breendavide alternativereferencespaceforhecolornormalization AT garciafernandou alternativereferencespaceforhecolornormalization |