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Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study
Age related macular degeneration (AMD) is the leading cause of blindness among people aged 50 and over. Retinal deposition of amyloid-β (Aβ) aggregates in AMD patients has suggested a potential link between AMD and Alzheimer's disease (AD). We have evaluated the differential retinal expression...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371655/ https://www.ncbi.nlm.nih.gov/pubmed/28424619 http://dx.doi.org/10.3389/fphar.2017.00168 |
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author | Romano, Giovanni L. Platania, Chiara B. M. Drago, Filippo Salomone, Salvatore Ragusa, Marco Barbagallo, Cristina Di Pietro, Cinzia Purrello, Michele Reibaldi, Michele Avitabile, Teresio Longo, Antonio Bucolo, Claudio |
author_facet | Romano, Giovanni L. Platania, Chiara B. M. Drago, Filippo Salomone, Salvatore Ragusa, Marco Barbagallo, Cristina Di Pietro, Cinzia Purrello, Michele Reibaldi, Michele Avitabile, Teresio Longo, Antonio Bucolo, Claudio |
author_sort | Romano, Giovanni L. |
collection | PubMed |
description | Age related macular degeneration (AMD) is the leading cause of blindness among people aged 50 and over. Retinal deposition of amyloid-β (Aβ) aggregates in AMD patients has suggested a potential link between AMD and Alzheimer's disease (AD). We have evaluated the differential retinal expression profile of miRNAs in a rat model of AMD elicited by Aβ. A serum profile of miRNAs in AMD patients has been also assessed using single TaqMan assay. Analysis of retina from rats intravitreally injected with Aβ revealed that miR-27a, miR-146a, and miR-155 were up-regulated in comparison to control rats. Seven miRNA (miR-9, miR-23a, miR-27a, miR-34a, miR-126, miR-146a, and miR-155) have been found to be dysregulated in serum of AMD patients in comparison to control group. Analysis of pathways has revealed that dysregulated miRNAs, both in the AMD animal model and in AMD patients, can target genes regulating pathways linked to neurodegeneration and inflammation, reinforcing the hypothesis that AMD is a protein misfolding disease similar to AD. In fact, miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 have been found to be dysregulated both in AMD and AD. In conclusion, we suggest that miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 represent potential biomarkers and new pharmacological targets for AMD. |
format | Online Article Text |
id | pubmed-5371655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53716552017-04-19 Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study Romano, Giovanni L. Platania, Chiara B. M. Drago, Filippo Salomone, Salvatore Ragusa, Marco Barbagallo, Cristina Di Pietro, Cinzia Purrello, Michele Reibaldi, Michele Avitabile, Teresio Longo, Antonio Bucolo, Claudio Front Pharmacol Pharmacology Age related macular degeneration (AMD) is the leading cause of blindness among people aged 50 and over. Retinal deposition of amyloid-β (Aβ) aggregates in AMD patients has suggested a potential link between AMD and Alzheimer's disease (AD). We have evaluated the differential retinal expression profile of miRNAs in a rat model of AMD elicited by Aβ. A serum profile of miRNAs in AMD patients has been also assessed using single TaqMan assay. Analysis of retina from rats intravitreally injected with Aβ revealed that miR-27a, miR-146a, and miR-155 were up-regulated in comparison to control rats. Seven miRNA (miR-9, miR-23a, miR-27a, miR-34a, miR-126, miR-146a, and miR-155) have been found to be dysregulated in serum of AMD patients in comparison to control group. Analysis of pathways has revealed that dysregulated miRNAs, both in the AMD animal model and in AMD patients, can target genes regulating pathways linked to neurodegeneration and inflammation, reinforcing the hypothesis that AMD is a protein misfolding disease similar to AD. In fact, miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 have been found to be dysregulated both in AMD and AD. In conclusion, we suggest that miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 represent potential biomarkers and new pharmacological targets for AMD. Frontiers Media S.A. 2017-03-30 /pmc/articles/PMC5371655/ /pubmed/28424619 http://dx.doi.org/10.3389/fphar.2017.00168 Text en Copyright © 2017 Romano, Platania, Drago, Salomone, Ragusa, Barbagallo, Di Pietro, Purrello, Reibaldi, Avitabile, Longo and Bucolo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Romano, Giovanni L. Platania, Chiara B. M. Drago, Filippo Salomone, Salvatore Ragusa, Marco Barbagallo, Cristina Di Pietro, Cinzia Purrello, Michele Reibaldi, Michele Avitabile, Teresio Longo, Antonio Bucolo, Claudio Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study |
title | Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study |
title_full | Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study |
title_fullStr | Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study |
title_full_unstemmed | Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study |
title_short | Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study |
title_sort | retinal and circulating mirnas in age-related macular degeneration: an in vivo animal and human study |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371655/ https://www.ncbi.nlm.nih.gov/pubmed/28424619 http://dx.doi.org/10.3389/fphar.2017.00168 |
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