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Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes

Pollen tubes grow by spatially and temporally regulated expansion of new material secreted into the cell wall at the tip of the tube. A complex web of interactions among cellular components, ions and small molecule provides dynamic control of localized expansion and secretion. Cross-correlation stud...

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Autores principales: Winship, Lawrence J., Rounds, Caleb, Hepler, Peter K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371762/
https://www.ncbi.nlm.nih.gov/pubmed/28042810
http://dx.doi.org/10.3390/plants6010003
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author Winship, Lawrence J.
Rounds, Caleb
Hepler, Peter K.
author_facet Winship, Lawrence J.
Rounds, Caleb
Hepler, Peter K.
author_sort Winship, Lawrence J.
collection PubMed
description Pollen tubes grow by spatially and temporally regulated expansion of new material secreted into the cell wall at the tip of the tube. A complex web of interactions among cellular components, ions and small molecule provides dynamic control of localized expansion and secretion. Cross-correlation studies on oscillating lily (Lilium formosanum Wallace) pollen tubes showed that an increase in intracellular calcium follows an increase in growth, whereas the increase in the alkaline band and in secretion both anticipate the increase in growth rate. Calcium, as a follower, is unlikely to be a stimulator of growth, whereas the alkaline band, as a leader, may be an activator. To gain further insight herein we reversibly inhibited growth with potassium cyanide (KCN) and followed the re-establishment of calcium, pH and secretion patterns as growth resumed. While KCN markedly slows growth and causes the associated gradients of calcium and pH to sharply decline, its removal allows growth and vital processes to fully recover. The calcium gradient reappears before growth restarts; however, it is preceded by both the alkaline band and secretion, in which the alkaline band is slightly advanced over secretion. Thus the pH gradient, rather than the tip-focused calcium gradient, may regulate pollen tube growth.
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spelling pubmed-53717622017-04-10 Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes Winship, Lawrence J. Rounds, Caleb Hepler, Peter K. Plants (Basel) Article Pollen tubes grow by spatially and temporally regulated expansion of new material secreted into the cell wall at the tip of the tube. A complex web of interactions among cellular components, ions and small molecule provides dynamic control of localized expansion and secretion. Cross-correlation studies on oscillating lily (Lilium formosanum Wallace) pollen tubes showed that an increase in intracellular calcium follows an increase in growth, whereas the increase in the alkaline band and in secretion both anticipate the increase in growth rate. Calcium, as a follower, is unlikely to be a stimulator of growth, whereas the alkaline band, as a leader, may be an activator. To gain further insight herein we reversibly inhibited growth with potassium cyanide (KCN) and followed the re-establishment of calcium, pH and secretion patterns as growth resumed. While KCN markedly slows growth and causes the associated gradients of calcium and pH to sharply decline, its removal allows growth and vital processes to fully recover. The calcium gradient reappears before growth restarts; however, it is preceded by both the alkaline band and secretion, in which the alkaline band is slightly advanced over secretion. Thus the pH gradient, rather than the tip-focused calcium gradient, may regulate pollen tube growth. MDPI 2016-12-30 /pmc/articles/PMC5371762/ /pubmed/28042810 http://dx.doi.org/10.3390/plants6010003 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Winship, Lawrence J.
Rounds, Caleb
Hepler, Peter K.
Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes
title Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes
title_full Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes
title_fullStr Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes
title_full_unstemmed Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes
title_short Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes
title_sort perturbation analysis of calcium, alkalinity and secretion during growth of lily pollen tubes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371762/
https://www.ncbi.nlm.nih.gov/pubmed/28042810
http://dx.doi.org/10.3390/plants6010003
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