Cargando…

High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)

Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of bi...

Descripción completa

Detalles Bibliográficos
Autores principales: Gasmi, Leila, Ferré, Juan, Herrero, Salvador
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371785/
https://www.ncbi.nlm.nih.gov/pubmed/28257054
http://dx.doi.org/10.3390/bios7010012
_version_ 1782518491355545600
author Gasmi, Leila
Ferré, Juan
Herrero, Salvador
author_facet Gasmi, Leila
Ferré, Juan
Herrero, Salvador
author_sort Gasmi, Leila
collection PubMed
description Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the Lepidoptera Spodoptera exigua were produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ and Gram− as well as their carbohydrate binding specificities was determined for the three lectins. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as a biosensor or for other biotechnological applications involving bacterial capture.
format Online
Article
Text
id pubmed-5371785
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-53717852017-04-10 High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae) Gasmi, Leila Ferré, Juan Herrero, Salvador Biosensors (Basel) Article Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the Lepidoptera Spodoptera exigua were produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ and Gram− as well as their carbohydrate binding specificities was determined for the three lectins. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as a biosensor or for other biotechnological applications involving bacterial capture. MDPI 2017-03-01 /pmc/articles/PMC5371785/ /pubmed/28257054 http://dx.doi.org/10.3390/bios7010012 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gasmi, Leila
Ferré, Juan
Herrero, Salvador
High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
title High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
title_full High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
title_fullStr High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
title_full_unstemmed High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
title_short High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
title_sort high bacterial agglutination activity in a single-crd c-type lectin from spodoptera exigua (lepidoptera: noctuidae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371785/
https://www.ncbi.nlm.nih.gov/pubmed/28257054
http://dx.doi.org/10.3390/bios7010012
work_keys_str_mv AT gasmileila highbacterialagglutinationactivityinasinglecrdctypelectinfromspodopteraexigualepidopteranoctuidae
AT ferrejuan highbacterialagglutinationactivityinasinglecrdctypelectinfromspodopteraexigualepidopteranoctuidae
AT herrerosalvador highbacterialagglutinationactivityinasinglecrdctypelectinfromspodopteraexigualepidopteranoctuidae