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A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups

The pathogenicity of the different flu species is a real public health problem worldwide. To combat this scourge, we established a method to detect drug targets, reducing the possibility of escape. Besides being able to attach a drug candidate, these targets should have the main characteristic of be...

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Detalles Bibliográficos
Autores principales: Lao, Julie, Vanet, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371793/
https://www.ncbi.nlm.nih.gov/pubmed/28257108
http://dx.doi.org/10.3390/v9030038
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author Lao, Julie
Vanet, Anne
author_facet Lao, Julie
Vanet, Anne
author_sort Lao, Julie
collection PubMed
description The pathogenicity of the different flu species is a real public health problem worldwide. To combat this scourge, we established a method to detect drug targets, reducing the possibility of escape. Besides being able to attach a drug candidate, these targets should have the main characteristic of being part of an essential viral function. The invariance groups that are sets of residues bearing an essential function can be detected genetically. They consist of invariant and synthetic lethal residues (interdependent residues not varying or slightly varying when together). We analyzed an alignment of more than 10,000 hemagglutinin sequences of influenza to detect six invariance groups, close in space, and on the protein surface. In parallel we identified five potential pockets on the surface of hemagglutinin. By combining these results, three potential binding sites were determined that are composed of invariance groups located respectively in the vestigial esterase domain, in the bottom of the stem and in the fusion area. The latter target is constituted of residues involved in the spring-loaded mechanism, an essential step in the fusion process. We propose a model describing how this potential target could block the reorganization of the hemagglutinin HA2 secondary structure and prevent viral entry into the host cell.
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spelling pubmed-53717932017-04-10 A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups Lao, Julie Vanet, Anne Viruses Article The pathogenicity of the different flu species is a real public health problem worldwide. To combat this scourge, we established a method to detect drug targets, reducing the possibility of escape. Besides being able to attach a drug candidate, these targets should have the main characteristic of being part of an essential viral function. The invariance groups that are sets of residues bearing an essential function can be detected genetically. They consist of invariant and synthetic lethal residues (interdependent residues not varying or slightly varying when together). We analyzed an alignment of more than 10,000 hemagglutinin sequences of influenza to detect six invariance groups, close in space, and on the protein surface. In parallel we identified five potential pockets on the surface of hemagglutinin. By combining these results, three potential binding sites were determined that are composed of invariance groups located respectively in the vestigial esterase domain, in the bottom of the stem and in the fusion area. The latter target is constituted of residues involved in the spring-loaded mechanism, an essential step in the fusion process. We propose a model describing how this potential target could block the reorganization of the hemagglutinin HA2 secondary structure and prevent viral entry into the host cell. MDPI 2017-03-02 /pmc/articles/PMC5371793/ /pubmed/28257108 http://dx.doi.org/10.3390/v9030038 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lao, Julie
Vanet, Anne
A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups
title A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups
title_full A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups
title_fullStr A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups
title_full_unstemmed A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups
title_short A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups
title_sort new strategy to reduce influenza escape: detecting therapeutic targets constituted of invariance groups
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371793/
https://www.ncbi.nlm.nih.gov/pubmed/28257108
http://dx.doi.org/10.3390/v9030038
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