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Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells

Piscine orthoreovirus (PRV) is ubiquitous in farmed Atlantic salmon (Salmo salar) and the cause of heart and skeletal muscle inflammation. Erythrocytes are important target cells for PRV. We have investigated the kinetics of PRV infection in salmon blood cells. The findings indicate that PRV causes...

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Autores principales: Haatveit, Hanne Merethe, Wessel, Øystein, Markussen, Turhan, Lund, Morten, Thiede, Bernd, Nyman, Ingvild Berg, Braaen, Stine, Dahle, Maria Krudtaa, Rimstad, Espen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371804/
https://www.ncbi.nlm.nih.gov/pubmed/28335455
http://dx.doi.org/10.3390/v9030049
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author Haatveit, Hanne Merethe
Wessel, Øystein
Markussen, Turhan
Lund, Morten
Thiede, Bernd
Nyman, Ingvild Berg
Braaen, Stine
Dahle, Maria Krudtaa
Rimstad, Espen
author_facet Haatveit, Hanne Merethe
Wessel, Øystein
Markussen, Turhan
Lund, Morten
Thiede, Bernd
Nyman, Ingvild Berg
Braaen, Stine
Dahle, Maria Krudtaa
Rimstad, Espen
author_sort Haatveit, Hanne Merethe
collection PubMed
description Piscine orthoreovirus (PRV) is ubiquitous in farmed Atlantic salmon (Salmo salar) and the cause of heart and skeletal muscle inflammation. Erythrocytes are important target cells for PRV. We have investigated the kinetics of PRV infection in salmon blood cells. The findings indicate that PRV causes an acute infection of blood cells lasting 1–2 weeks, before it subsides into persistence. A high production of viral proteins occurred initially in the acute phase which significantly correlated with antiviral gene transcription. Globular viral factories organized by the non-structural protein µNS were also observed initially, but were not evident at later stages. Interactions between µNS and the PRV structural proteins λ1, µ1, σ1 and σ3 were demonstrated. Different size variants of µNS and the outer capsid protein µ1 appeared at specific time points during infection. Maximal viral protein load was observed five weeks post cohabitant challenge and was undetectable from seven weeks post challenge. In contrast, viral RNA at a high level could be detected throughout the eight-week trial. A proteolytic cleavage fragment of the µ1 protein was the only viral protein detectable after seven weeks post challenge, indicating that this µ1 fragment may be involved in the mechanisms of persistent infection.
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spelling pubmed-53718042017-04-10 Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells Haatveit, Hanne Merethe Wessel, Øystein Markussen, Turhan Lund, Morten Thiede, Bernd Nyman, Ingvild Berg Braaen, Stine Dahle, Maria Krudtaa Rimstad, Espen Viruses Article Piscine orthoreovirus (PRV) is ubiquitous in farmed Atlantic salmon (Salmo salar) and the cause of heart and skeletal muscle inflammation. Erythrocytes are important target cells for PRV. We have investigated the kinetics of PRV infection in salmon blood cells. The findings indicate that PRV causes an acute infection of blood cells lasting 1–2 weeks, before it subsides into persistence. A high production of viral proteins occurred initially in the acute phase which significantly correlated with antiviral gene transcription. Globular viral factories organized by the non-structural protein µNS were also observed initially, but were not evident at later stages. Interactions between µNS and the PRV structural proteins λ1, µ1, σ1 and σ3 were demonstrated. Different size variants of µNS and the outer capsid protein µ1 appeared at specific time points during infection. Maximal viral protein load was observed five weeks post cohabitant challenge and was undetectable from seven weeks post challenge. In contrast, viral RNA at a high level could be detected throughout the eight-week trial. A proteolytic cleavage fragment of the µ1 protein was the only viral protein detectable after seven weeks post challenge, indicating that this µ1 fragment may be involved in the mechanisms of persistent infection. MDPI 2017-03-18 /pmc/articles/PMC5371804/ /pubmed/28335455 http://dx.doi.org/10.3390/v9030049 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Haatveit, Hanne Merethe
Wessel, Øystein
Markussen, Turhan
Lund, Morten
Thiede, Bernd
Nyman, Ingvild Berg
Braaen, Stine
Dahle, Maria Krudtaa
Rimstad, Espen
Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells
title Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells
title_full Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells
title_fullStr Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells
title_full_unstemmed Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells
title_short Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells
title_sort viral protein kinetics of piscine orthoreovirus infection in atlantic salmon blood cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371804/
https://www.ncbi.nlm.nih.gov/pubmed/28335455
http://dx.doi.org/10.3390/v9030049
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