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Antivenom Evaluation by Electrophysiological Analysis
Scorpion stings on humans are medically relevant because they may contain toxins that specifically target ion channels. During antivenom production, pharmaceutical companies must use a large number of experimental animals to ensure the antivenom’s efficacy according to pharmacopeia methods. Here we...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371829/ https://www.ncbi.nlm.nih.gov/pubmed/28241514 http://dx.doi.org/10.3390/toxins9030074 |
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author | Restano-Cassulini, Rita Garcia, Walter Paniagua-Solís, Jorge F. Possani, Lourival D. |
author_facet | Restano-Cassulini, Rita Garcia, Walter Paniagua-Solís, Jorge F. Possani, Lourival D. |
author_sort | Restano-Cassulini, Rita |
collection | PubMed |
description | Scorpion stings on humans are medically relevant because they may contain toxins that specifically target ion channels. During antivenom production, pharmaceutical companies must use a large number of experimental animals to ensure the antivenom’s efficacy according to pharmacopeia methods. Here we present an electrophysiological alternative for the evaluation of horse antivenoms produced against two species of Moroccan scorpions: Buthus mardochei and Androctonus mauretanicus. Human sodium and potassium channels and acetylcholine nicotinic receptors were analyzed by standard patch-clamp techniques. The results showed that the antivenom is capable of reversing ion current disruption caused by the venom application. We propose the use of this in vitro technique for antivenom evaluation as an alternative to using a large number of live animals. |
format | Online Article Text |
id | pubmed-5371829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53718292017-04-10 Antivenom Evaluation by Electrophysiological Analysis Restano-Cassulini, Rita Garcia, Walter Paniagua-Solís, Jorge F. Possani, Lourival D. Toxins (Basel) Article Scorpion stings on humans are medically relevant because they may contain toxins that specifically target ion channels. During antivenom production, pharmaceutical companies must use a large number of experimental animals to ensure the antivenom’s efficacy according to pharmacopeia methods. Here we present an electrophysiological alternative for the evaluation of horse antivenoms produced against two species of Moroccan scorpions: Buthus mardochei and Androctonus mauretanicus. Human sodium and potassium channels and acetylcholine nicotinic receptors were analyzed by standard patch-clamp techniques. The results showed that the antivenom is capable of reversing ion current disruption caused by the venom application. We propose the use of this in vitro technique for antivenom evaluation as an alternative to using a large number of live animals. MDPI 2017-02-23 /pmc/articles/PMC5371829/ /pubmed/28241514 http://dx.doi.org/10.3390/toxins9030074 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Restano-Cassulini, Rita Garcia, Walter Paniagua-Solís, Jorge F. Possani, Lourival D. Antivenom Evaluation by Electrophysiological Analysis |
title | Antivenom Evaluation by Electrophysiological Analysis |
title_full | Antivenom Evaluation by Electrophysiological Analysis |
title_fullStr | Antivenom Evaluation by Electrophysiological Analysis |
title_full_unstemmed | Antivenom Evaluation by Electrophysiological Analysis |
title_short | Antivenom Evaluation by Electrophysiological Analysis |
title_sort | antivenom evaluation by electrophysiological analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371829/ https://www.ncbi.nlm.nih.gov/pubmed/28241514 http://dx.doi.org/10.3390/toxins9030074 |
work_keys_str_mv | AT restanocassulinirita antivenomevaluationbyelectrophysiologicalanalysis AT garciawalter antivenomevaluationbyelectrophysiologicalanalysis AT paniaguasolisjorgef antivenomevaluationbyelectrophysiologicalanalysis AT possanilourivald antivenomevaluationbyelectrophysiologicalanalysis |