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Antivenom Evaluation by Electrophysiological Analysis

Scorpion stings on humans are medically relevant because they may contain toxins that specifically target ion channels. During antivenom production, pharmaceutical companies must use a large number of experimental animals to ensure the antivenom’s efficacy according to pharmacopeia methods. Here we...

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Detalles Bibliográficos
Autores principales: Restano-Cassulini, Rita, Garcia, Walter, Paniagua-Solís, Jorge F., Possani, Lourival D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371829/
https://www.ncbi.nlm.nih.gov/pubmed/28241514
http://dx.doi.org/10.3390/toxins9030074
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author Restano-Cassulini, Rita
Garcia, Walter
Paniagua-Solís, Jorge F.
Possani, Lourival D.
author_facet Restano-Cassulini, Rita
Garcia, Walter
Paniagua-Solís, Jorge F.
Possani, Lourival D.
author_sort Restano-Cassulini, Rita
collection PubMed
description Scorpion stings on humans are medically relevant because they may contain toxins that specifically target ion channels. During antivenom production, pharmaceutical companies must use a large number of experimental animals to ensure the antivenom’s efficacy according to pharmacopeia methods. Here we present an electrophysiological alternative for the evaluation of horse antivenoms produced against two species of Moroccan scorpions: Buthus mardochei and Androctonus mauretanicus. Human sodium and potassium channels and acetylcholine nicotinic receptors were analyzed by standard patch-clamp techniques. The results showed that the antivenom is capable of reversing ion current disruption caused by the venom application. We propose the use of this in vitro technique for antivenom evaluation as an alternative to using a large number of live animals.
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spelling pubmed-53718292017-04-10 Antivenom Evaluation by Electrophysiological Analysis Restano-Cassulini, Rita Garcia, Walter Paniagua-Solís, Jorge F. Possani, Lourival D. Toxins (Basel) Article Scorpion stings on humans are medically relevant because they may contain toxins that specifically target ion channels. During antivenom production, pharmaceutical companies must use a large number of experimental animals to ensure the antivenom’s efficacy according to pharmacopeia methods. Here we present an electrophysiological alternative for the evaluation of horse antivenoms produced against two species of Moroccan scorpions: Buthus mardochei and Androctonus mauretanicus. Human sodium and potassium channels and acetylcholine nicotinic receptors were analyzed by standard patch-clamp techniques. The results showed that the antivenom is capable of reversing ion current disruption caused by the venom application. We propose the use of this in vitro technique for antivenom evaluation as an alternative to using a large number of live animals. MDPI 2017-02-23 /pmc/articles/PMC5371829/ /pubmed/28241514 http://dx.doi.org/10.3390/toxins9030074 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Restano-Cassulini, Rita
Garcia, Walter
Paniagua-Solís, Jorge F.
Possani, Lourival D.
Antivenom Evaluation by Electrophysiological Analysis
title Antivenom Evaluation by Electrophysiological Analysis
title_full Antivenom Evaluation by Electrophysiological Analysis
title_fullStr Antivenom Evaluation by Electrophysiological Analysis
title_full_unstemmed Antivenom Evaluation by Electrophysiological Analysis
title_short Antivenom Evaluation by Electrophysiological Analysis
title_sort antivenom evaluation by electrophysiological analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371829/
https://www.ncbi.nlm.nih.gov/pubmed/28241514
http://dx.doi.org/10.3390/toxins9030074
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