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Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis

The ability to selectively amplify the detection signals for targets over interferences is crucial when analyzing proteins in a complicated sample matrix. Here, we describe a targeted enlargement strategy that can amplify the light-scattering signal from aptamer-functionalized gold nanoparticles (Ap...

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Detalles Bibliográficos
Autores principales: Li, Feng, Li, Jingjing, Tang, Yanan, Wang, Chuan, Li, Xing-Fang, Le, X. Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372222/
https://www.ncbi.nlm.nih.gov/pubmed/28248252
http://dx.doi.org/10.3390/proteomes5010001
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author Li, Feng
Li, Jingjing
Tang, Yanan
Wang, Chuan
Li, Xing-Fang
Le, X. Chris
author_facet Li, Feng
Li, Jingjing
Tang, Yanan
Wang, Chuan
Li, Xing-Fang
Le, X. Chris
author_sort Li, Feng
collection PubMed
description The ability to selectively amplify the detection signals for targets over interferences is crucial when analyzing proteins in a complicated sample matrix. Here, we describe a targeted enlargement strategy that can amplify the light-scattering signal from aptamer-functionalized gold nanoparticles (Apt-AuNP) with high specificity for quantitative protein analysis. This strategy is achieved by labeling target proteins with competitively protected Apt-AuNP probes and enlarging the probes with gold enhancement. This competitive protection strategy could effectively eliminate nonspecific protein adsorptions from a sample matrix, leading to a highly specific labeling of the target protein. As a result, the subsequent amplification of the light-scattering signal by gold enhancement only occurs in the presence of the target protein. This strategy was successfully demonstrated by analyzing human α-thrombin in human serum samples in a Western blot format.
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spelling pubmed-53722222017-04-05 Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis Li, Feng Li, Jingjing Tang, Yanan Wang, Chuan Li, Xing-Fang Le, X. Chris Proteomes Article The ability to selectively amplify the detection signals for targets over interferences is crucial when analyzing proteins in a complicated sample matrix. Here, we describe a targeted enlargement strategy that can amplify the light-scattering signal from aptamer-functionalized gold nanoparticles (Apt-AuNP) with high specificity for quantitative protein analysis. This strategy is achieved by labeling target proteins with competitively protected Apt-AuNP probes and enlarging the probes with gold enhancement. This competitive protection strategy could effectively eliminate nonspecific protein adsorptions from a sample matrix, leading to a highly specific labeling of the target protein. As a result, the subsequent amplification of the light-scattering signal by gold enhancement only occurs in the presence of the target protein. This strategy was successfully demonstrated by analyzing human α-thrombin in human serum samples in a Western blot format. MDPI 2016-12-22 /pmc/articles/PMC5372222/ /pubmed/28248252 http://dx.doi.org/10.3390/proteomes5010001 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Feng
Li, Jingjing
Tang, Yanan
Wang, Chuan
Li, Xing-Fang
Le, X. Chris
Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis
title Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis
title_full Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis
title_fullStr Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis
title_full_unstemmed Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis
title_short Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis
title_sort targeted enlargement of aptamer functionalized gold nanoparticles for quantitative protein analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372222/
https://www.ncbi.nlm.nih.gov/pubmed/28248252
http://dx.doi.org/10.3390/proteomes5010001
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