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Isoelectric Point Separations of Peptides and Proteins

The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and con...

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Autores principales: Pergande, Melissa R., Cologna, Stephanie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372225/
https://www.ncbi.nlm.nih.gov/pubmed/28248255
http://dx.doi.org/10.3390/proteomes5010004
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author Pergande, Melissa R.
Cologna, Stephanie M.
author_facet Pergande, Melissa R.
Cologna, Stephanie M.
author_sort Pergande, Melissa R.
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description The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies.
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spelling pubmed-53722252017-04-05 Isoelectric Point Separations of Peptides and Proteins Pergande, Melissa R. Cologna, Stephanie M. Proteomes Review The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies. MDPI 2017-01-25 /pmc/articles/PMC5372225/ /pubmed/28248255 http://dx.doi.org/10.3390/proteomes5010004 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pergande, Melissa R.
Cologna, Stephanie M.
Isoelectric Point Separations of Peptides and Proteins
title Isoelectric Point Separations of Peptides and Proteins
title_full Isoelectric Point Separations of Peptides and Proteins
title_fullStr Isoelectric Point Separations of Peptides and Proteins
title_full_unstemmed Isoelectric Point Separations of Peptides and Proteins
title_short Isoelectric Point Separations of Peptides and Proteins
title_sort isoelectric point separations of peptides and proteins
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372225/
https://www.ncbi.nlm.nih.gov/pubmed/28248255
http://dx.doi.org/10.3390/proteomes5010004
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