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Isoelectric Point Separations of Peptides and Proteins
The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and con...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372225/ https://www.ncbi.nlm.nih.gov/pubmed/28248255 http://dx.doi.org/10.3390/proteomes5010004 |
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author | Pergande, Melissa R. Cologna, Stephanie M. |
author_facet | Pergande, Melissa R. Cologna, Stephanie M. |
author_sort | Pergande, Melissa R. |
collection | PubMed |
description | The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies. |
format | Online Article Text |
id | pubmed-5372225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53722252017-04-05 Isoelectric Point Separations of Peptides and Proteins Pergande, Melissa R. Cologna, Stephanie M. Proteomes Review The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies. MDPI 2017-01-25 /pmc/articles/PMC5372225/ /pubmed/28248255 http://dx.doi.org/10.3390/proteomes5010004 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pergande, Melissa R. Cologna, Stephanie M. Isoelectric Point Separations of Peptides and Proteins |
title | Isoelectric Point Separations of Peptides and Proteins |
title_full | Isoelectric Point Separations of Peptides and Proteins |
title_fullStr | Isoelectric Point Separations of Peptides and Proteins |
title_full_unstemmed | Isoelectric Point Separations of Peptides and Proteins |
title_short | Isoelectric Point Separations of Peptides and Proteins |
title_sort | isoelectric point separations of peptides and proteins |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372225/ https://www.ncbi.nlm.nih.gov/pubmed/28248255 http://dx.doi.org/10.3390/proteomes5010004 |
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