Cargando…
The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats
Objective: To investigate the pharmacological effects of Leonuri Herba alkaloids (LHA) on prostate hyperplasia in older rats and the effect mechanism. Methods: Remove bilateral testes from BPH model rats, and conduct subcutaneous injection of testosterone and estradiol. At the same time, feed corres...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372384/ https://www.ncbi.nlm.nih.gov/pubmed/28386189 http://dx.doi.org/10.1016/j.sjbs.2017.01.035 |
_version_ | 1782518604493750272 |
---|---|
author | Miao, Mingsan Wang, Tan Liu, Jing Li, Yan Fu, Zhenna Tian, Shuo |
author_facet | Miao, Mingsan Wang, Tan Liu, Jing Li, Yan Fu, Zhenna Tian, Shuo |
author_sort | Miao, Mingsan |
collection | PubMed |
description | Objective: To investigate the pharmacological effects of Leonuri Herba alkaloids (LHA) on prostate hyperplasia in older rats and the effect mechanism. Methods: Remove bilateral testes from BPH model rats, and conduct subcutaneous injection of testosterone and estradiol. At the same time, feed corresponding drugs to the rats by gastric perfusion for 30d. In the first 27d, conduct bladder fistula surgery. Three days after feeding, carry out the detection of the urine flow dynamics. Eyeball blood taking, determination of serum E2 levels, and quickly remove the prostate, thymus gland, spleen, kidney, lung, and bladder. 1/3 prostate homogenate, determine the level of PACP, T, DHT. 1/3 prostate was determined by mRNA expression in bFGF. The remaining 1/3 prostate was observed by light microscopy. Results: LHA could significantly decrease the animal prostate index, level of DHT, T, PACP, and elevate levels of E2 in the serum. It could also significantly reduce the maximum voiding pressure, intercontraction interval, and bladder resting pressure. Conclusion: LHA has good therapeutic effect on prostatic hyperplasia model rats induced by male and female hormone. |
format | Online Article Text |
id | pubmed-5372384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-53723842017-04-06 The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats Miao, Mingsan Wang, Tan Liu, Jing Li, Yan Fu, Zhenna Tian, Shuo Saudi J Biol Sci Original Article Objective: To investigate the pharmacological effects of Leonuri Herba alkaloids (LHA) on prostate hyperplasia in older rats and the effect mechanism. Methods: Remove bilateral testes from BPH model rats, and conduct subcutaneous injection of testosterone and estradiol. At the same time, feed corresponding drugs to the rats by gastric perfusion for 30d. In the first 27d, conduct bladder fistula surgery. Three days after feeding, carry out the detection of the urine flow dynamics. Eyeball blood taking, determination of serum E2 levels, and quickly remove the prostate, thymus gland, spleen, kidney, lung, and bladder. 1/3 prostate homogenate, determine the level of PACP, T, DHT. 1/3 prostate was determined by mRNA expression in bFGF. The remaining 1/3 prostate was observed by light microscopy. Results: LHA could significantly decrease the animal prostate index, level of DHT, T, PACP, and elevate levels of E2 in the serum. It could also significantly reduce the maximum voiding pressure, intercontraction interval, and bladder resting pressure. Conclusion: LHA has good therapeutic effect on prostatic hyperplasia model rats induced by male and female hormone. Elsevier 2017-03 2017-01-26 /pmc/articles/PMC5372384/ /pubmed/28386189 http://dx.doi.org/10.1016/j.sjbs.2017.01.035 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Miao, Mingsan Wang, Tan Liu, Jing Li, Yan Fu, Zhenna Tian, Shuo The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats |
title | The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats |
title_full | The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats |
title_fullStr | The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats |
title_full_unstemmed | The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats |
title_short | The Effect of Leonuri Herba alkaloids on Senile BPH (male and female hormone induced) model rats |
title_sort | effect of leonuri herba alkaloids on senile bph (male and female hormone induced) model rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372384/ https://www.ncbi.nlm.nih.gov/pubmed/28386189 http://dx.doi.org/10.1016/j.sjbs.2017.01.035 |
work_keys_str_mv | AT miaomingsan theeffectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT wangtan theeffectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT liujing theeffectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT liyan theeffectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT fuzhenna theeffectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT tianshuo theeffectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT miaomingsan effectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT wangtan effectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT liujing effectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT liyan effectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT fuzhenna effectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats AT tianshuo effectofleonuriherbaalkaloidsonsenilebphmaleandfemalehormoneinducedmodelrats |