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Optimizing conditions for calcium phosphate mediated transient transfection
BACKGROUND: Calcium phosphate mediated transfection has been used for delivering DNA into mammalian cells in excess of 30 years due to its most low cost for introducing recombinant DNA into culture cells. However, multiple factors affecting the transfect efficiency are commonly recognized meanwhile...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372392/ https://www.ncbi.nlm.nih.gov/pubmed/28386188 http://dx.doi.org/10.1016/j.sjbs.2017.01.034 |