Method for detection of hydrogen peroxide in HT22 cells

We have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is b...

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Detalles Bibliográficos
Autores principales: Jacewicz, Dagmara, Siedlecka-Kroplewska, Kamila, Drzeżdżon, Joanna, Piotrowska, Agnieszka, Wyrzykowski, Dariusz, Tesmar, Aleksandra, Żamojć, Krzysztof, Chmurzyński, Lech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372458/
https://www.ncbi.nlm.nih.gov/pubmed/28358356
http://dx.doi.org/10.1038/srep45673
Descripción
Sumario:We have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is based on two crucial reagents cis-[Cr(C(2)O(4))(pm)(OH(2))(2)](+) (pm denotes pyridoxamine) and 2-ketobutyrate. The results obtained indicate that the presented method can be a promising tool to detect hydrogen peroxide in biological samples, particularly in cellular experimental models.

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