Method for detection of hydrogen peroxide in HT22 cells

We have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is b...

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Autores principales: Jacewicz, Dagmara, Siedlecka-Kroplewska, Kamila, Drzeżdżon, Joanna, Piotrowska, Agnieszka, Wyrzykowski, Dariusz, Tesmar, Aleksandra, Żamojć, Krzysztof, Chmurzyński, Lech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372458/
https://www.ncbi.nlm.nih.gov/pubmed/28358356
http://dx.doi.org/10.1038/srep45673
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author Jacewicz, Dagmara
Siedlecka-Kroplewska, Kamila
Drzeżdżon, Joanna
Piotrowska, Agnieszka
Wyrzykowski, Dariusz
Tesmar, Aleksandra
Żamojć, Krzysztof
Chmurzyński, Lech
author_facet Jacewicz, Dagmara
Siedlecka-Kroplewska, Kamila
Drzeżdżon, Joanna
Piotrowska, Agnieszka
Wyrzykowski, Dariusz
Tesmar, Aleksandra
Żamojć, Krzysztof
Chmurzyński, Lech
author_sort Jacewicz, Dagmara
collection PubMed
description We have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is based on two crucial reagents cis-[Cr(C(2)O(4))(pm)(OH(2))(2)](+) (pm denotes pyridoxamine) and 2-ketobutyrate. The results obtained indicate that the presented method can be a promising tool to detect hydrogen peroxide in biological samples, particularly in cellular experimental models.
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spelling pubmed-53724582017-03-31 Method for detection of hydrogen peroxide in HT22 cells Jacewicz, Dagmara Siedlecka-Kroplewska, Kamila Drzeżdżon, Joanna Piotrowska, Agnieszka Wyrzykowski, Dariusz Tesmar, Aleksandra Żamojć, Krzysztof Chmurzyński, Lech Sci Rep Article We have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is based on two crucial reagents cis-[Cr(C(2)O(4))(pm)(OH(2))(2)](+) (pm denotes pyridoxamine) and 2-ketobutyrate. The results obtained indicate that the presented method can be a promising tool to detect hydrogen peroxide in biological samples, particularly in cellular experimental models. Nature Publishing Group 2017-03-30 /pmc/articles/PMC5372458/ /pubmed/28358356 http://dx.doi.org/10.1038/srep45673 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jacewicz, Dagmara
Siedlecka-Kroplewska, Kamila
Drzeżdżon, Joanna
Piotrowska, Agnieszka
Wyrzykowski, Dariusz
Tesmar, Aleksandra
Żamojć, Krzysztof
Chmurzyński, Lech
Method for detection of hydrogen peroxide in HT22 cells
title Method for detection of hydrogen peroxide in HT22 cells
title_full Method for detection of hydrogen peroxide in HT22 cells
title_fullStr Method for detection of hydrogen peroxide in HT22 cells
title_full_unstemmed Method for detection of hydrogen peroxide in HT22 cells
title_short Method for detection of hydrogen peroxide in HT22 cells
title_sort method for detection of hydrogen peroxide in ht22 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372458/
https://www.ncbi.nlm.nih.gov/pubmed/28358356
http://dx.doi.org/10.1038/srep45673
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