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Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study

Benign prostatic hyperplasia (BPH) treatment includes the apoptosis machinery modulation through the direct inhibition of caspase cascade. We previously demonstrated that Serenoa repens (Ser) with lycopene (Ly) and selenium (Se) reawakened apoptosis by reducing survivin and neuronal apoptosis inhibi...

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Autores principales: Morgia, Giuseppe, Micali, Antonio, Rinaldi, Mariagrazia, Irrera, Natasha, Marini, Herbert, Puzzolo, Domenico, Pisani, Antonina, Privitera, Salvatore, Russo, Giorgio I., Cimino, Sebastiano, Ieni, Antonio, Trichilo, Vincenzo, Altavilla, Domenica, Squadrito, Francesco, Minutoli, Letteria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372690/
https://www.ncbi.nlm.nih.gov/pubmed/28327526
http://dx.doi.org/10.3390/ijms18030680
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author Morgia, Giuseppe
Micali, Antonio
Rinaldi, Mariagrazia
Irrera, Natasha
Marini, Herbert
Puzzolo, Domenico
Pisani, Antonina
Privitera, Salvatore
Russo, Giorgio I.
Cimino, Sebastiano
Ieni, Antonio
Trichilo, Vincenzo
Altavilla, Domenica
Squadrito, Francesco
Minutoli, Letteria
author_facet Morgia, Giuseppe
Micali, Antonio
Rinaldi, Mariagrazia
Irrera, Natasha
Marini, Herbert
Puzzolo, Domenico
Pisani, Antonina
Privitera, Salvatore
Russo, Giorgio I.
Cimino, Sebastiano
Ieni, Antonio
Trichilo, Vincenzo
Altavilla, Domenica
Squadrito, Francesco
Minutoli, Letteria
author_sort Morgia, Giuseppe
collection PubMed
description Benign prostatic hyperplasia (BPH) treatment includes the apoptosis machinery modulation through the direct inhibition of caspase cascade. We previously demonstrated that Serenoa repens (Ser) with lycopene (Ly) and selenium (Se) reawakened apoptosis by reducing survivin and neuronal apoptosis inhibitory protein (NAIP) levels in rats. The aim of this study was to evaluate the effectiveness of Ser-Se-Ly association on survivin and NAIP expression in BPH patients. Ninety patients with lower urinary tract symptoms (LUTS) due to clinical BPH were included in this randomized, double-blind, placebo-controlled trial. Participants were randomly assigned to receive placebo (Group BPH + placebo, n = 45) or Ser-Se-Ly association (Group BPH + Ser-Se-Ly; n = 45) for 3 months. At time 0, all patients underwent prostatic biopsies. After 3 months of treatment, they underwent prostatic re-biopsy and specimens were collected for molecular, morphological, and immunohistochemical analysis. After 3 months, survivin and NAIP were significantly decreased, while caspase-3 was significantly increased in BPH patients treated with Ser-Se-Ly when compared with the other group. In BPH patients treated with Ser-Se-Ly for 3 months, the glandular epithelium was formed by a single layer of cuboidal cells. PSA showed high immunoexpression in all BPH patients and a focal positivity in Ser-Se-Ly treated patients after 3 months. Evident prostate specific membrane antigen (PSMA) immunoexpression was shown in all BPH patients, while no positivity was present after Ser-Se-Ly administration. Ser-Se-Ly proved to be effective in promoting apoptosis in BPH patients.
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spelling pubmed-53726902017-04-10 Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study Morgia, Giuseppe Micali, Antonio Rinaldi, Mariagrazia Irrera, Natasha Marini, Herbert Puzzolo, Domenico Pisani, Antonina Privitera, Salvatore Russo, Giorgio I. Cimino, Sebastiano Ieni, Antonio Trichilo, Vincenzo Altavilla, Domenica Squadrito, Francesco Minutoli, Letteria Int J Mol Sci Article Benign prostatic hyperplasia (BPH) treatment includes the apoptosis machinery modulation through the direct inhibition of caspase cascade. We previously demonstrated that Serenoa repens (Ser) with lycopene (Ly) and selenium (Se) reawakened apoptosis by reducing survivin and neuronal apoptosis inhibitory protein (NAIP) levels in rats. The aim of this study was to evaluate the effectiveness of Ser-Se-Ly association on survivin and NAIP expression in BPH patients. Ninety patients with lower urinary tract symptoms (LUTS) due to clinical BPH were included in this randomized, double-blind, placebo-controlled trial. Participants were randomly assigned to receive placebo (Group BPH + placebo, n = 45) or Ser-Se-Ly association (Group BPH + Ser-Se-Ly; n = 45) for 3 months. At time 0, all patients underwent prostatic biopsies. After 3 months of treatment, they underwent prostatic re-biopsy and specimens were collected for molecular, morphological, and immunohistochemical analysis. After 3 months, survivin and NAIP were significantly decreased, while caspase-3 was significantly increased in BPH patients treated with Ser-Se-Ly when compared with the other group. In BPH patients treated with Ser-Se-Ly for 3 months, the glandular epithelium was formed by a single layer of cuboidal cells. PSA showed high immunoexpression in all BPH patients and a focal positivity in Ser-Se-Ly treated patients after 3 months. Evident prostate specific membrane antigen (PSMA) immunoexpression was shown in all BPH patients, while no positivity was present after Ser-Se-Ly administration. Ser-Se-Ly proved to be effective in promoting apoptosis in BPH patients. MDPI 2017-03-22 /pmc/articles/PMC5372690/ /pubmed/28327526 http://dx.doi.org/10.3390/ijms18030680 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morgia, Giuseppe
Micali, Antonio
Rinaldi, Mariagrazia
Irrera, Natasha
Marini, Herbert
Puzzolo, Domenico
Pisani, Antonina
Privitera, Salvatore
Russo, Giorgio I.
Cimino, Sebastiano
Ieni, Antonio
Trichilo, Vincenzo
Altavilla, Domenica
Squadrito, Francesco
Minutoli, Letteria
Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study
title Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study
title_full Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study
title_fullStr Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study
title_full_unstemmed Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study
title_short Survivin and NAIP in Human Benign Prostatic Hyperplasia: Protective Role of the Association of Serenoa repens, Lycopene and Selenium from the Randomized Clinical Study
title_sort survivin and naip in human benign prostatic hyperplasia: protective role of the association of serenoa repens, lycopene and selenium from the randomized clinical study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372690/
https://www.ncbi.nlm.nih.gov/pubmed/28327526
http://dx.doi.org/10.3390/ijms18030680
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