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Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits

Probiotics such as Lactobacillus and Bifidobacterium have been successfully used to promote growth and prevent diseases. Previous reports have demonstrated that Bacillus subtilis (B. subtilis) was a potential probiotic for animals. In this research, 180 B. subtilis were isolated from the soil, ident...

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Autores principales: Guo, Mengjiao, Wu, Fahao, Hao, Guangen, Qi, Qin, Li, Rong, Li, Ning, Wei, Liangmeng, Chai, Tongjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372816/
https://www.ncbi.nlm.nih.gov/pubmed/28424690
http://dx.doi.org/10.3389/fimmu.2017.00354
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author Guo, Mengjiao
Wu, Fahao
Hao, Guangen
Qi, Qin
Li, Rong
Li, Ning
Wei, Liangmeng
Chai, Tongjie
author_facet Guo, Mengjiao
Wu, Fahao
Hao, Guangen
Qi, Qin
Li, Rong
Li, Ning
Wei, Liangmeng
Chai, Tongjie
author_sort Guo, Mengjiao
collection PubMed
description Probiotics such as Lactobacillus and Bifidobacterium have been successfully used to promote growth and prevent diseases. Previous reports have demonstrated that Bacillus subtilis (B. subtilis) was a potential probiotic for animals. In this research, 180 B. subtilis were isolated from the soil, identified, and investigated in vitro. Furthermore, five B. subtilis were selected and mixed to investigate their effect on growth performance, immune response, intestine microbiota, and disease resistance in rabbits. Rabbits with a diet of 10(6) CFU g(−1) mixed B. subtilis exhibited the best growth performance and higher serum IgG and IgA than controls (P < 0.05). Moreover, dairy with B. subtilis can promote the balance of intestinal flora. The major proinflammatory factor and β-defensin were upregulated compared with the controls. After 7 weeks of feeding, the survival rate of the rabbits fed with B. subtilis was significantly higher than those in the control groups postinfected with Escherichia coli. At the same time, this study detected higher expression of β-defensin and reduced bacteria contents of the heart and cecal contents from the diet mixed with B. subtilis compared with the control groups. In conclusion, dietary supplementation with B. subtilis for rabbits could improve growth performance, intestinal homeostasis, and immune organ index and enhance innate immune response as well as disease resistance. These findings showed that the induction of β-defensin by B. subtilis might be an interesting new therapeutic strategy to strengthen innate defense mechanisms.
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spelling pubmed-53728162017-04-19 Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits Guo, Mengjiao Wu, Fahao Hao, Guangen Qi, Qin Li, Rong Li, Ning Wei, Liangmeng Chai, Tongjie Front Immunol Immunology Probiotics such as Lactobacillus and Bifidobacterium have been successfully used to promote growth and prevent diseases. Previous reports have demonstrated that Bacillus subtilis (B. subtilis) was a potential probiotic for animals. In this research, 180 B. subtilis were isolated from the soil, identified, and investigated in vitro. Furthermore, five B. subtilis were selected and mixed to investigate their effect on growth performance, immune response, intestine microbiota, and disease resistance in rabbits. Rabbits with a diet of 10(6) CFU g(−1) mixed B. subtilis exhibited the best growth performance and higher serum IgG and IgA than controls (P < 0.05). Moreover, dairy with B. subtilis can promote the balance of intestinal flora. The major proinflammatory factor and β-defensin were upregulated compared with the controls. After 7 weeks of feeding, the survival rate of the rabbits fed with B. subtilis was significantly higher than those in the control groups postinfected with Escherichia coli. At the same time, this study detected higher expression of β-defensin and reduced bacteria contents of the heart and cecal contents from the diet mixed with B. subtilis compared with the control groups. In conclusion, dietary supplementation with B. subtilis for rabbits could improve growth performance, intestinal homeostasis, and immune organ index and enhance innate immune response as well as disease resistance. These findings showed that the induction of β-defensin by B. subtilis might be an interesting new therapeutic strategy to strengthen innate defense mechanisms. Frontiers Media S.A. 2017-03-29 /pmc/articles/PMC5372816/ /pubmed/28424690 http://dx.doi.org/10.3389/fimmu.2017.00354 Text en Copyright © 2017 Guo, Wu, Hao, Qi, Li, Li, Wei and Chai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Guo, Mengjiao
Wu, Fahao
Hao, Guangen
Qi, Qin
Li, Rong
Li, Ning
Wei, Liangmeng
Chai, Tongjie
Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits
title Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits
title_full Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits
title_fullStr Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits
title_full_unstemmed Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits
title_short Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits
title_sort bacillus subtilis improves immunity and disease resistance in rabbits
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372816/
https://www.ncbi.nlm.nih.gov/pubmed/28424690
http://dx.doi.org/10.3389/fimmu.2017.00354
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