Cargando…
Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang
Exploring traditional medicines may lead to the development of low-cost and non-toxic cancer preventive agents. Si-Wu-Tang (SWT), comprising the combination of four herbs, Rehmanniae, Angelica, Chuanxiong, and Paeoniae, is one of the most popular traditional Chinese medicines for women’s diseases. I...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372963/ https://www.ncbi.nlm.nih.gov/pubmed/28335476 http://dx.doi.org/10.3390/nu9030300 |
_version_ | 1782518728014954496 |
---|---|
author | Liu, Mandy M. Huang, Kevin M. Yeung, Steven Chang, Andy Zhang, Suhui Mei, Nan Parsa, Cyrus Orlando, Robert Huang, Ying |
author_facet | Liu, Mandy M. Huang, Kevin M. Yeung, Steven Chang, Andy Zhang, Suhui Mei, Nan Parsa, Cyrus Orlando, Robert Huang, Ying |
author_sort | Liu, Mandy M. |
collection | PubMed |
description | Exploring traditional medicines may lead to the development of low-cost and non-toxic cancer preventive agents. Si-Wu-Tang (SWT), comprising the combination of four herbs, Rehmanniae, Angelica, Chuanxiong, and Paeoniae, is one of the most popular traditional Chinese medicines for women’s diseases. In our previous studies, the antioxidant Nrf2 pathways were strongly induced by SWT in vitro and in vivo. Since Nrf2 activation has been associated with anticarcinogenic effects, the purpose of this study is to evaluate SWT’s activity of cancer prevention. In the Ames test, SWT demonstrated an antimutagenic activity against mutagenicity induced by the chemical carcinogen 7,12-dimethylbenz(a)anthracene (DMBA). In JB6 P+ cells, a non-cancerous murine epidermal model for studying tumor promotion, SWT inhibited epidermal growth factor (EGF)-induced neoplastic transformation. The luciferase reporter gene assays demonstrated that SWT suppressed EGF-induced AP-1 and TNF-α-induced NF-κB activation, which are essential factors involved in skin carcinogenesis. In a DMBA-induced skin hyperplasia assay in ‘Sensitivity to Carcinogenesis’ (SENCAR) mice, both topical and oral SWT inhibited DMBA-induced epidermal hyperplasia, expression of the proliferation marker Proliferating cell nuclear antigen (PCNA), and H-ras mutations. These findings demonstrate, for the first time, that SWT prevents tumor promoter and chemical-induced carcinogenesis in vitro and in vivo, partly by inhibiting DNA damage and blocking the activation of AP-1 and NF-κB. |
format | Online Article Text |
id | pubmed-5372963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53729632017-04-05 Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang Liu, Mandy M. Huang, Kevin M. Yeung, Steven Chang, Andy Zhang, Suhui Mei, Nan Parsa, Cyrus Orlando, Robert Huang, Ying Nutrients Article Exploring traditional medicines may lead to the development of low-cost and non-toxic cancer preventive agents. Si-Wu-Tang (SWT), comprising the combination of four herbs, Rehmanniae, Angelica, Chuanxiong, and Paeoniae, is one of the most popular traditional Chinese medicines for women’s diseases. In our previous studies, the antioxidant Nrf2 pathways were strongly induced by SWT in vitro and in vivo. Since Nrf2 activation has been associated with anticarcinogenic effects, the purpose of this study is to evaluate SWT’s activity of cancer prevention. In the Ames test, SWT demonstrated an antimutagenic activity against mutagenicity induced by the chemical carcinogen 7,12-dimethylbenz(a)anthracene (DMBA). In JB6 P+ cells, a non-cancerous murine epidermal model for studying tumor promotion, SWT inhibited epidermal growth factor (EGF)-induced neoplastic transformation. The luciferase reporter gene assays demonstrated that SWT suppressed EGF-induced AP-1 and TNF-α-induced NF-κB activation, which are essential factors involved in skin carcinogenesis. In a DMBA-induced skin hyperplasia assay in ‘Sensitivity to Carcinogenesis’ (SENCAR) mice, both topical and oral SWT inhibited DMBA-induced epidermal hyperplasia, expression of the proliferation marker Proliferating cell nuclear antigen (PCNA), and H-ras mutations. These findings demonstrate, for the first time, that SWT prevents tumor promoter and chemical-induced carcinogenesis in vitro and in vivo, partly by inhibiting DNA damage and blocking the activation of AP-1 and NF-κB. MDPI 2017-03-18 /pmc/articles/PMC5372963/ /pubmed/28335476 http://dx.doi.org/10.3390/nu9030300 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Mandy M. Huang, Kevin M. Yeung, Steven Chang, Andy Zhang, Suhui Mei, Nan Parsa, Cyrus Orlando, Robert Huang, Ying Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang |
title | Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang |
title_full | Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang |
title_fullStr | Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang |
title_full_unstemmed | Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang |
title_short | Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang |
title_sort | inhibition of neoplastic transformation and chemically-induced skin hyperplasia in mice by traditional chinese medicinal formula si-wu-tang |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372963/ https://www.ncbi.nlm.nih.gov/pubmed/28335476 http://dx.doi.org/10.3390/nu9030300 |
work_keys_str_mv | AT liumandym inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT huangkevinm inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT yeungsteven inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT changandy inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT zhangsuhui inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT meinan inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT parsacyrus inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT orlandorobert inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang AT huangying inhibitionofneoplastictransformationandchemicallyinducedskinhyperplasiainmicebytraditionalchinesemedicinalformulasiwutang |