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A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection

Background: Over the next 20 years, oropharyngeal cancers (OPC) will represent the majority of head and neck cancers (HNCs) in the United States. It is estimated that human papillomavirus (HPV) may account for as much as 70% to 80% of OPCs in North America and in certain parts of Europe. It is hence...

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Autores principales: Sun, Charles Xiaohang, Bennett, Nigel, Tran, Peter, Tang, Kai Dun, Lim, Yenkai, Frazer, Ian, Samaranayake, Lakshman, Punyadeera, Chamindie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373020/
https://www.ncbi.nlm.nih.gov/pubmed/28257064
http://dx.doi.org/10.3390/diagnostics7010011
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author Sun, Charles Xiaohang
Bennett, Nigel
Tran, Peter
Tang, Kai Dun
Lim, Yenkai
Frazer, Ian
Samaranayake, Lakshman
Punyadeera, Chamindie
author_facet Sun, Charles Xiaohang
Bennett, Nigel
Tran, Peter
Tang, Kai Dun
Lim, Yenkai
Frazer, Ian
Samaranayake, Lakshman
Punyadeera, Chamindie
author_sort Sun, Charles Xiaohang
collection PubMed
description Background: Over the next 20 years, oropharyngeal cancers (OPC) will represent the majority of head and neck cancers (HNCs) in the United States. It is estimated that human papillomavirus (HPV) may account for as much as 70% to 80% of OPCs in North America and in certain parts of Europe. It is hence crucial to understand the disease risk factors and natural history of oral HPV infections. We hypothesized that poor oral health (by measures such as poor oral hygiene and periodontal disease) leads to a higher degree of oral HPV-16 infections within a patient cohort from a dental school clinic. This study aims to test this hypothesis and gauge possible disease associations before larger scale studies. Subjects and Methods: 223 participants were recruited in this study from the University of Queensland Dental School clinic. Clinical oral health parameters (such as oral hygiene measures and periodontal disease measurements) have been examined and determined by dental professionals. We have collected oral rinse samples from these volunteers. Results: 10 (4.5%) out of 223 participants were found to have HPV-16 DNA in their oral rinse samples using NB2 endpoint PCR and Sanger sequencing. Within the HPV-16 DNA positive subjects, 7 (70%) and 3 (30%) were associated with poor oral hygiene and periodontal disease, respectively. Conclusion: Our results show a trend towards a positive correlation between oral HPV-16 infection and poor clinical oral health status.
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spelling pubmed-53730202017-04-05 A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection Sun, Charles Xiaohang Bennett, Nigel Tran, Peter Tang, Kai Dun Lim, Yenkai Frazer, Ian Samaranayake, Lakshman Punyadeera, Chamindie Diagnostics (Basel) Article Background: Over the next 20 years, oropharyngeal cancers (OPC) will represent the majority of head and neck cancers (HNCs) in the United States. It is estimated that human papillomavirus (HPV) may account for as much as 70% to 80% of OPCs in North America and in certain parts of Europe. It is hence crucial to understand the disease risk factors and natural history of oral HPV infections. We hypothesized that poor oral health (by measures such as poor oral hygiene and periodontal disease) leads to a higher degree of oral HPV-16 infections within a patient cohort from a dental school clinic. This study aims to test this hypothesis and gauge possible disease associations before larger scale studies. Subjects and Methods: 223 participants were recruited in this study from the University of Queensland Dental School clinic. Clinical oral health parameters (such as oral hygiene measures and periodontal disease measurements) have been examined and determined by dental professionals. We have collected oral rinse samples from these volunteers. Results: 10 (4.5%) out of 223 participants were found to have HPV-16 DNA in their oral rinse samples using NB2 endpoint PCR and Sanger sequencing. Within the HPV-16 DNA positive subjects, 7 (70%) and 3 (30%) were associated with poor oral hygiene and periodontal disease, respectively. Conclusion: Our results show a trend towards a positive correlation between oral HPV-16 infection and poor clinical oral health status. MDPI 2017-03-01 /pmc/articles/PMC5373020/ /pubmed/28257064 http://dx.doi.org/10.3390/diagnostics7010011 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Charles Xiaohang
Bennett, Nigel
Tran, Peter
Tang, Kai Dun
Lim, Yenkai
Frazer, Ian
Samaranayake, Lakshman
Punyadeera, Chamindie
A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection
title A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection
title_full A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection
title_fullStr A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection
title_full_unstemmed A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection
title_short A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection
title_sort pilot study into the association between oral health status and human papillomavirus—16 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373020/
https://www.ncbi.nlm.nih.gov/pubmed/28257064
http://dx.doi.org/10.3390/diagnostics7010011
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