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Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq
BACKGROUND: Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373514/ https://www.ncbi.nlm.nih.gov/pubmed/28358880 http://dx.doi.org/10.1371/journal.pntd.0005357 |
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author | Grote, Alexandra Voronin, Denis Ding, Tao Twaddle, Alan Unnasch, Thomas R. Lustigman, Sara Ghedin, Elodie |
author_facet | Grote, Alexandra Voronin, Denis Ding, Tao Twaddle, Alan Unnasch, Thomas R. Lustigman, Sara Ghedin, Elodie |
author_sort | Grote, Alexandra |
collection | PubMed |
description | BACKGROUND: Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial parasite that can be maintained in small laboratory animals. Many filarial nematode species, including B. malayi, carry an obligate endosymbiont, the alpha-proteobacteria Wolbachia, which can be eliminated through antibiotic treatment. Elimination of the endosymbiont interferes with development, reproduction, and survival of the worms within the mamalian host, a clear indicator that the Wolbachia are crucial for survival of the parasite. Little is understood about the mechanism underlying this symbiosis. METHODOLOGY/ PRINCIPLE FINDINGS: To better understand the molecular interplay between these two organisms we profiled the transcriptomes of B. malayi and Wolbachia by dual RNA-seq across the life cycle of the parasite. This helped identify functional pathways involved in this essential symbiotic relationship provided by the co-expression of nematode and bacterial genes. We have identified significant stage-specific and gender-specific differential expression in Wolbachia during the nematode’s development. For example, during female worm development we find that Wolbachia upregulate genes involved in ATP production and purine biosynthesis, as well as genes involved in the oxidative stress response. CONCLUSIONS/ SIGNIFICANCE: This global transcriptional analysis has highlighted specific pathways to which both Wolbachia and B. malayi contribute concurrently over the life cycle of the parasite, paving the way for the development of novel intervention strategies. |
format | Online Article Text |
id | pubmed-5373514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53735142017-04-07 Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq Grote, Alexandra Voronin, Denis Ding, Tao Twaddle, Alan Unnasch, Thomas R. Lustigman, Sara Ghedin, Elodie PLoS Negl Trop Dis Research Article BACKGROUND: Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial parasite that can be maintained in small laboratory animals. Many filarial nematode species, including B. malayi, carry an obligate endosymbiont, the alpha-proteobacteria Wolbachia, which can be eliminated through antibiotic treatment. Elimination of the endosymbiont interferes with development, reproduction, and survival of the worms within the mamalian host, a clear indicator that the Wolbachia are crucial for survival of the parasite. Little is understood about the mechanism underlying this symbiosis. METHODOLOGY/ PRINCIPLE FINDINGS: To better understand the molecular interplay between these two organisms we profiled the transcriptomes of B. malayi and Wolbachia by dual RNA-seq across the life cycle of the parasite. This helped identify functional pathways involved in this essential symbiotic relationship provided by the co-expression of nematode and bacterial genes. We have identified significant stage-specific and gender-specific differential expression in Wolbachia during the nematode’s development. For example, during female worm development we find that Wolbachia upregulate genes involved in ATP production and purine biosynthesis, as well as genes involved in the oxidative stress response. CONCLUSIONS/ SIGNIFICANCE: This global transcriptional analysis has highlighted specific pathways to which both Wolbachia and B. malayi contribute concurrently over the life cycle of the parasite, paving the way for the development of novel intervention strategies. Public Library of Science 2017-03-30 /pmc/articles/PMC5373514/ /pubmed/28358880 http://dx.doi.org/10.1371/journal.pntd.0005357 Text en © 2017 Grote et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Grote, Alexandra Voronin, Denis Ding, Tao Twaddle, Alan Unnasch, Thomas R. Lustigman, Sara Ghedin, Elodie Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq |
title | Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq |
title_full | Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq |
title_fullStr | Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq |
title_full_unstemmed | Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq |
title_short | Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq |
title_sort | defining brugia malayi and wolbachia symbiosis by stage-specific dual rna-seq |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373514/ https://www.ncbi.nlm.nih.gov/pubmed/28358880 http://dx.doi.org/10.1371/journal.pntd.0005357 |
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