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Stroke and Circulating Extracellular RNAs
BACKGROUND AND PURPOSE—: There is increasing interest in extracellular RNAs (ex-RNAs), with numerous reports of associations between selected microRNAs (miRNAs) and a variety of cardiovascular disease phenotypes. Previous studies of ex-RNAs in relation to risk for cardiovascular disease have investi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373984/ https://www.ncbi.nlm.nih.gov/pubmed/28289238 http://dx.doi.org/10.1161/STROKEAHA.116.015140 |
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author | Mick, Eric Shah, Ravi Tanriverdi, Kahraman Murthy, Venkatesh Gerstein, Mark Rozowsky, Joel Kitchen, Robert Larson, Martin G. Levy, Daniel Freedman, Jane E. |
author_facet | Mick, Eric Shah, Ravi Tanriverdi, Kahraman Murthy, Venkatesh Gerstein, Mark Rozowsky, Joel Kitchen, Robert Larson, Martin G. Levy, Daniel Freedman, Jane E. |
author_sort | Mick, Eric |
collection | PubMed |
description | BACKGROUND AND PURPOSE—: There is increasing interest in extracellular RNAs (ex-RNAs), with numerous reports of associations between selected microRNAs (miRNAs) and a variety of cardiovascular disease phenotypes. Previous studies of ex-RNAs in relation to risk for cardiovascular disease have investigated small numbers of patients and assayed only candidate miRNAs. No human studies have investigated links between novel ex-RNAs and stroke. METHODS—: We conducted unbiased next-generation sequencing using plasma from 40 participants of the FHS (Framingham Heart Study; Offspring Cohort Exam 8) followed by high-throughput polymerase chain reaction of 471 ex-RNAs. The reverse transcription quantitative polymerase chain reaction included 331 of the most abundant miRNAs, 43 small nucleolar RNAs, and 97 piwi-interacting RNAs in 2763 additional FHS participants and explored the relations of ex-RNAs and prevalent (n=63) and incident (n=51) stroke and coronary heart disease (prevalent=286, incident=69). RESULTS—: After adjustment for multiple cardiovascular disease risk factors, 7 ex-RNAs were associated with stroke prevalence or incidence; there were no ex-RNA associated with prevalent or incident coronary heart disease. Statistically significant ex-RNA associations with stroke were specific, with no overlap between prevalent and incident events. CONCLUSIONS—: This is the largest study of ex-RNAs in relation to stroke using an unbiased approach in an observational cohort and the first large study to examine human small noncoding RNAs beyond miRNAs. These results demonstrate that when studied in a large observational cohort, extracellular miRNAs are associated with stroke risk. |
format | Online Article Text |
id | pubmed-5373984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-53739842017-08-09 Stroke and Circulating Extracellular RNAs Mick, Eric Shah, Ravi Tanriverdi, Kahraman Murthy, Venkatesh Gerstein, Mark Rozowsky, Joel Kitchen, Robert Larson, Martin G. Levy, Daniel Freedman, Jane E. Stroke Original Contributions BACKGROUND AND PURPOSE—: There is increasing interest in extracellular RNAs (ex-RNAs), with numerous reports of associations between selected microRNAs (miRNAs) and a variety of cardiovascular disease phenotypes. Previous studies of ex-RNAs in relation to risk for cardiovascular disease have investigated small numbers of patients and assayed only candidate miRNAs. No human studies have investigated links between novel ex-RNAs and stroke. METHODS—: We conducted unbiased next-generation sequencing using plasma from 40 participants of the FHS (Framingham Heart Study; Offspring Cohort Exam 8) followed by high-throughput polymerase chain reaction of 471 ex-RNAs. The reverse transcription quantitative polymerase chain reaction included 331 of the most abundant miRNAs, 43 small nucleolar RNAs, and 97 piwi-interacting RNAs in 2763 additional FHS participants and explored the relations of ex-RNAs and prevalent (n=63) and incident (n=51) stroke and coronary heart disease (prevalent=286, incident=69). RESULTS—: After adjustment for multiple cardiovascular disease risk factors, 7 ex-RNAs were associated with stroke prevalence or incidence; there were no ex-RNA associated with prevalent or incident coronary heart disease. Statistically significant ex-RNA associations with stroke were specific, with no overlap between prevalent and incident events. CONCLUSIONS—: This is the largest study of ex-RNAs in relation to stroke using an unbiased approach in an observational cohort and the first large study to examine human small noncoding RNAs beyond miRNAs. These results demonstrate that when studied in a large observational cohort, extracellular miRNAs are associated with stroke risk. Lippincott Williams & Wilkins 2017-04 2017-03-27 /pmc/articles/PMC5373984/ /pubmed/28289238 http://dx.doi.org/10.1161/STROKEAHA.116.015140 Text en © 2017 The Authors. Stroke is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made. |
spellingShingle | Original Contributions Mick, Eric Shah, Ravi Tanriverdi, Kahraman Murthy, Venkatesh Gerstein, Mark Rozowsky, Joel Kitchen, Robert Larson, Martin G. Levy, Daniel Freedman, Jane E. Stroke and Circulating Extracellular RNAs |
title | Stroke and Circulating Extracellular RNAs |
title_full | Stroke and Circulating Extracellular RNAs |
title_fullStr | Stroke and Circulating Extracellular RNAs |
title_full_unstemmed | Stroke and Circulating Extracellular RNAs |
title_short | Stroke and Circulating Extracellular RNAs |
title_sort | stroke and circulating extracellular rnas |
topic | Original Contributions |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373984/ https://www.ncbi.nlm.nih.gov/pubmed/28289238 http://dx.doi.org/10.1161/STROKEAHA.116.015140 |
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