Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci

Cas9-mediated, high-throughput, saturating in situ mutagenesis permits fine-mapping of function across genomic segments. Disease- and trait-associated variants from genome-wide association studies largely cluster in regulatory DNA. Here we demonstrate the use of multiple designer nucleases and varia...

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Detalles Bibliográficos
Autores principales: Canver, Matthew C., Lessard, Samuel, Pinello, Luca, Wu, Yuxuan, Ilboudo, Yann, Stern, Emily N., Needleman, Austen J., Galactéros, Frédéric, Brugnara, Carlo, Kutlar, Abdullah, McKenzie, Colin, Reid, Marvin, Chen, Diane D., Das, Partha Pratim, Cole, Mitchel, Zeng, Jing, Kurita, Ryo, Nakamura, Yukio, Yuan, Guo-Cheng, Lettre, Guillaume, Bauer, Daniel E., Orkin, Stuart H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374001/
https://www.ncbi.nlm.nih.gov/pubmed/28218758
http://dx.doi.org/10.1038/ng.3793

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