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One-Step Optogenetics with Multifunctional Flexible Polymer Fibers

Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities within a s...

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Autores principales: Park, Seongjun, Guo, Yuanyuan, Jia, Xiaoting, Choe, Han Kyoung, Grena, Benjamin, Kang, Jeewoo, Park, Jiyeon, Lu, Chi, Canales, Andres, Chen, Ritchie, Yim, Yeong Shin, Choi, Gloria B., Fink, Yoel, Anikeeva, Polina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374019/
https://www.ncbi.nlm.nih.gov/pubmed/28218915
http://dx.doi.org/10.1038/nn.4510
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author Park, Seongjun
Guo, Yuanyuan
Jia, Xiaoting
Choe, Han Kyoung
Grena, Benjamin
Kang, Jeewoo
Park, Jiyeon
Lu, Chi
Canales, Andres
Chen, Ritchie
Yim, Yeong Shin
Choi, Gloria B.
Fink, Yoel
Anikeeva, Polina
author_facet Park, Seongjun
Guo, Yuanyuan
Jia, Xiaoting
Choe, Han Kyoung
Grena, Benjamin
Kang, Jeewoo
Park, Jiyeon
Lu, Chi
Canales, Andres
Chen, Ritchie
Yim, Yeong Shin
Choi, Gloria B.
Fink, Yoel
Anikeeva, Polina
author_sort Park, Seongjun
collection PubMed
description Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities within a single biocompatible platform remains a challenge. Here, we introduce a device composed of an optical waveguide, six electrodes, and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes, while providing collocated neural recording and optical stimulation. The miniature (< 200 μm) footprint and modest weight (<0.5 g) of these probes allowed for multiple implantations into the mouse brain, which enabled opto-electrophysiological investigation of projections from the basolateral amygdala to the medial prefrontal cortex and ventral hippocampus during behavioral experiments. Fabricated solely from polymers and polymer composites, these flexible probes minimized tissue response to achieve chronic multimodal interrogation of brain circuits with high fidelity.
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spelling pubmed-53740192017-08-20 One-Step Optogenetics with Multifunctional Flexible Polymer Fibers Park, Seongjun Guo, Yuanyuan Jia, Xiaoting Choe, Han Kyoung Grena, Benjamin Kang, Jeewoo Park, Jiyeon Lu, Chi Canales, Andres Chen, Ritchie Yim, Yeong Shin Choi, Gloria B. Fink, Yoel Anikeeva, Polina Nat Neurosci Article Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities within a single biocompatible platform remains a challenge. Here, we introduce a device composed of an optical waveguide, six electrodes, and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes, while providing collocated neural recording and optical stimulation. The miniature (< 200 μm) footprint and modest weight (<0.5 g) of these probes allowed for multiple implantations into the mouse brain, which enabled opto-electrophysiological investigation of projections from the basolateral amygdala to the medial prefrontal cortex and ventral hippocampus during behavioral experiments. Fabricated solely from polymers and polymer composites, these flexible probes minimized tissue response to achieve chronic multimodal interrogation of brain circuits with high fidelity. 2017-02-20 2017-04 /pmc/articles/PMC5374019/ /pubmed/28218915 http://dx.doi.org/10.1038/nn.4510 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Park, Seongjun
Guo, Yuanyuan
Jia, Xiaoting
Choe, Han Kyoung
Grena, Benjamin
Kang, Jeewoo
Park, Jiyeon
Lu, Chi
Canales, Andres
Chen, Ritchie
Yim, Yeong Shin
Choi, Gloria B.
Fink, Yoel
Anikeeva, Polina
One-Step Optogenetics with Multifunctional Flexible Polymer Fibers
title One-Step Optogenetics with Multifunctional Flexible Polymer Fibers
title_full One-Step Optogenetics with Multifunctional Flexible Polymer Fibers
title_fullStr One-Step Optogenetics with Multifunctional Flexible Polymer Fibers
title_full_unstemmed One-Step Optogenetics with Multifunctional Flexible Polymer Fibers
title_short One-Step Optogenetics with Multifunctional Flexible Polymer Fibers
title_sort one-step optogenetics with multifunctional flexible polymer fibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374019/
https://www.ncbi.nlm.nih.gov/pubmed/28218915
http://dx.doi.org/10.1038/nn.4510
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