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One-Step Optogenetics with Multifunctional Flexible Polymer Fibers
Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities within a s...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374019/ https://www.ncbi.nlm.nih.gov/pubmed/28218915 http://dx.doi.org/10.1038/nn.4510 |
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author | Park, Seongjun Guo, Yuanyuan Jia, Xiaoting Choe, Han Kyoung Grena, Benjamin Kang, Jeewoo Park, Jiyeon Lu, Chi Canales, Andres Chen, Ritchie Yim, Yeong Shin Choi, Gloria B. Fink, Yoel Anikeeva, Polina |
author_facet | Park, Seongjun Guo, Yuanyuan Jia, Xiaoting Choe, Han Kyoung Grena, Benjamin Kang, Jeewoo Park, Jiyeon Lu, Chi Canales, Andres Chen, Ritchie Yim, Yeong Shin Choi, Gloria B. Fink, Yoel Anikeeva, Polina |
author_sort | Park, Seongjun |
collection | PubMed |
description | Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities within a single biocompatible platform remains a challenge. Here, we introduce a device composed of an optical waveguide, six electrodes, and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes, while providing collocated neural recording and optical stimulation. The miniature (< 200 μm) footprint and modest weight (<0.5 g) of these probes allowed for multiple implantations into the mouse brain, which enabled opto-electrophysiological investigation of projections from the basolateral amygdala to the medial prefrontal cortex and ventral hippocampus during behavioral experiments. Fabricated solely from polymers and polymer composites, these flexible probes minimized tissue response to achieve chronic multimodal interrogation of brain circuits with high fidelity. |
format | Online Article Text |
id | pubmed-5374019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
record_format | MEDLINE/PubMed |
spelling | pubmed-53740192017-08-20 One-Step Optogenetics with Multifunctional Flexible Polymer Fibers Park, Seongjun Guo, Yuanyuan Jia, Xiaoting Choe, Han Kyoung Grena, Benjamin Kang, Jeewoo Park, Jiyeon Lu, Chi Canales, Andres Chen, Ritchie Yim, Yeong Shin Choi, Gloria B. Fink, Yoel Anikeeva, Polina Nat Neurosci Article Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities within a single biocompatible platform remains a challenge. Here, we introduce a device composed of an optical waveguide, six electrodes, and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes, while providing collocated neural recording and optical stimulation. The miniature (< 200 μm) footprint and modest weight (<0.5 g) of these probes allowed for multiple implantations into the mouse brain, which enabled opto-electrophysiological investigation of projections from the basolateral amygdala to the medial prefrontal cortex and ventral hippocampus during behavioral experiments. Fabricated solely from polymers and polymer composites, these flexible probes minimized tissue response to achieve chronic multimodal interrogation of brain circuits with high fidelity. 2017-02-20 2017-04 /pmc/articles/PMC5374019/ /pubmed/28218915 http://dx.doi.org/10.1038/nn.4510 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Park, Seongjun Guo, Yuanyuan Jia, Xiaoting Choe, Han Kyoung Grena, Benjamin Kang, Jeewoo Park, Jiyeon Lu, Chi Canales, Andres Chen, Ritchie Yim, Yeong Shin Choi, Gloria B. Fink, Yoel Anikeeva, Polina One-Step Optogenetics with Multifunctional Flexible Polymer Fibers |
title | One-Step Optogenetics with Multifunctional Flexible Polymer Fibers |
title_full | One-Step Optogenetics with Multifunctional Flexible Polymer Fibers |
title_fullStr | One-Step Optogenetics with Multifunctional Flexible Polymer Fibers |
title_full_unstemmed | One-Step Optogenetics with Multifunctional Flexible Polymer Fibers |
title_short | One-Step Optogenetics with Multifunctional Flexible Polymer Fibers |
title_sort | one-step optogenetics with multifunctional flexible polymer fibers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374019/ https://www.ncbi.nlm.nih.gov/pubmed/28218915 http://dx.doi.org/10.1038/nn.4510 |
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