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Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark
Photosynthetic microorganisms such as cyanobacteria have attracted attention for their potential to produce biofuels and biochemicals directly from CO(2). Cell isolation by colony has conventionally been used for selecting target cells. Colony isolation methods require a significant amount of time f...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374263/ https://www.ncbi.nlm.nih.gov/pubmed/28435803 http://dx.doi.org/10.1016/j.btre.2014.10.003 |
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author | Arai, Sayuri Okochi, Mina Hanai, Taizo Honda, Hiroyuki |
author_facet | Arai, Sayuri Okochi, Mina Hanai, Taizo Honda, Hiroyuki |
author_sort | Arai, Sayuri |
collection | PubMed |
description | Photosynthetic microorganisms such as cyanobacteria have attracted attention for their potential to produce biofuels and biochemicals directly from CO(2). Cell isolation by colony has conventionally been used for selecting target cells. Colony isolation methods require a significant amount of time for cultivation, and the colony-forming ratio is potentially low for cyanobacteria. Here, we overcome such limitations by encapsulating and culturing cells in droplets with an overlay of dodecane using glass slides printed with highly water-repellent mark. In the compartmentalized culture, the oil phase protects the small volume of culture medium from drying and increases the CO(2) supply. Since a difference in cell growth was observed with and without the addition of antibiotics, this compartmentalized culture method could be a powerful tool for mutant selection. |
format | Online Article Text |
id | pubmed-5374263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-53742632017-04-21 Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark Arai, Sayuri Okochi, Mina Hanai, Taizo Honda, Hiroyuki Biotechnol Rep (Amst) Article Photosynthetic microorganisms such as cyanobacteria have attracted attention for their potential to produce biofuels and biochemicals directly from CO(2). Cell isolation by colony has conventionally been used for selecting target cells. Colony isolation methods require a significant amount of time for cultivation, and the colony-forming ratio is potentially low for cyanobacteria. Here, we overcome such limitations by encapsulating and culturing cells in droplets with an overlay of dodecane using glass slides printed with highly water-repellent mark. In the compartmentalized culture, the oil phase protects the small volume of culture medium from drying and increases the CO(2) supply. Since a difference in cell growth was observed with and without the addition of antibiotics, this compartmentalized culture method could be a powerful tool for mutant selection. Elsevier 2014-10-16 /pmc/articles/PMC5374263/ /pubmed/28435803 http://dx.doi.org/10.1016/j.btre.2014.10.003 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Article Arai, Sayuri Okochi, Mina Hanai, Taizo Honda, Hiroyuki Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark |
title | Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark |
title_full | Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark |
title_fullStr | Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark |
title_full_unstemmed | Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark |
title_short | Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark |
title_sort | micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374263/ https://www.ncbi.nlm.nih.gov/pubmed/28435803 http://dx.doi.org/10.1016/j.btre.2014.10.003 |
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