A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()

The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genom...

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Detalles Bibliográficos
Autores principales: Martins, Polyana Kelly, Ribeiro, Ana Paula, Cunha, Bárbara Andrade Dias Brito da, Kobayashi, Adilson Kenji, Molinari, Hugo Bruno Correa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374281/
https://www.ncbi.nlm.nih.gov/pubmed/28435807
http://dx.doi.org/10.1016/j.btre.2015.02.002
Descripción
Sumario:The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane.

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