A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()

The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genom...

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Autores principales: Martins, Polyana Kelly, Ribeiro, Ana Paula, Cunha, Bárbara Andrade Dias Brito da, Kobayashi, Adilson Kenji, Molinari, Hugo Bruno Correa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374281/
https://www.ncbi.nlm.nih.gov/pubmed/28435807
http://dx.doi.org/10.1016/j.btre.2015.02.002
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author Martins, Polyana Kelly
Ribeiro, Ana Paula
Cunha, Bárbara Andrade Dias Brito da
Kobayashi, Adilson Kenji
Molinari, Hugo Bruno Correa
author_facet Martins, Polyana Kelly
Ribeiro, Ana Paula
Cunha, Bárbara Andrade Dias Brito da
Kobayashi, Adilson Kenji
Molinari, Hugo Bruno Correa
author_sort Martins, Polyana Kelly
collection PubMed
description The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane.
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spelling pubmed-53742812017-04-21 A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis() Martins, Polyana Kelly Ribeiro, Ana Paula Cunha, Bárbara Andrade Dias Brito da Kobayashi, Adilson Kenji Molinari, Hugo Bruno Correa Biotechnol Rep (Amst) Short Communication The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane. Elsevier 2015-02-18 /pmc/articles/PMC5374281/ /pubmed/28435807 http://dx.doi.org/10.1016/j.btre.2015.02.002 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Martins, Polyana Kelly
Ribeiro, Ana Paula
Cunha, Bárbara Andrade Dias Brito da
Kobayashi, Adilson Kenji
Molinari, Hugo Bruno Correa
A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()
title A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()
title_full A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()
title_fullStr A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()
title_full_unstemmed A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()
title_short A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis()
title_sort simple and highly efficient agrobacterium-mediated transformation protocol for setaria viridis()
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374281/
https://www.ncbi.nlm.nih.gov/pubmed/28435807
http://dx.doi.org/10.1016/j.btre.2015.02.002
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