Cargando…

A novel mode of induction of the humoral innate immune response in Drosophila larvae

Drosophila adults have been utilized as a genetically tractable model organism to decipher the molecular mechanisms of humoral innate immune responses. In an effort to promote the utility of Drosophila larvae as an additional model system, in this study, we describe a novel aspect of an induction me...

Descripción completa

Detalles Bibliográficos
Autores principales: Kenmoku, Hiroyuki, Hori, Aki, Kuraishi, Takayuki, Kurata, Shoichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374318/
https://www.ncbi.nlm.nih.gov/pubmed/28250052
http://dx.doi.org/10.1242/dmm.027102
_version_ 1782518867778600960
author Kenmoku, Hiroyuki
Hori, Aki
Kuraishi, Takayuki
Kurata, Shoichiro
author_facet Kenmoku, Hiroyuki
Hori, Aki
Kuraishi, Takayuki
Kurata, Shoichiro
author_sort Kenmoku, Hiroyuki
collection PubMed
description Drosophila adults have been utilized as a genetically tractable model organism to decipher the molecular mechanisms of humoral innate immune responses. In an effort to promote the utility of Drosophila larvae as an additional model system, in this study, we describe a novel aspect of an induction mechanism for innate immunity in these larvae. By using a fine tungsten needle created for manipulating semi-conductor devices, larvae were subjected to septic injury. However, although Toll pathway mutants were susceptible to infection with Gram-positive bacteria as had been shown for Drosophila adults, microbe clearance was not affected in the mutants. In addition, Drosophila larvae were found to be sensitive to mechanical stimuli with respect to the activation of a sterile humoral response. In particular, pinching with forceps to a degree that might cause minor damage to larval tissues could induce the expression of the antifungal peptide gene Drosomycin; notably, this induction was partially independent of the Toll and immune deficiency pathways. We therefore propose that Drosophila larvae might serve as a useful model to analyze the infectious and non-infectious inflammation that underlies various inflammatory diseases such as ischemia, atherosclerosis and cancer.
format Online
Article
Text
id pubmed-5374318
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The Company of Biologists Ltd
record_format MEDLINE/PubMed
spelling pubmed-53743182017-04-10 A novel mode of induction of the humoral innate immune response in Drosophila larvae Kenmoku, Hiroyuki Hori, Aki Kuraishi, Takayuki Kurata, Shoichiro Dis Model Mech Research Article Drosophila adults have been utilized as a genetically tractable model organism to decipher the molecular mechanisms of humoral innate immune responses. In an effort to promote the utility of Drosophila larvae as an additional model system, in this study, we describe a novel aspect of an induction mechanism for innate immunity in these larvae. By using a fine tungsten needle created for manipulating semi-conductor devices, larvae were subjected to septic injury. However, although Toll pathway mutants were susceptible to infection with Gram-positive bacteria as had been shown for Drosophila adults, microbe clearance was not affected in the mutants. In addition, Drosophila larvae were found to be sensitive to mechanical stimuli with respect to the activation of a sterile humoral response. In particular, pinching with forceps to a degree that might cause minor damage to larval tissues could induce the expression of the antifungal peptide gene Drosomycin; notably, this induction was partially independent of the Toll and immune deficiency pathways. We therefore propose that Drosophila larvae might serve as a useful model to analyze the infectious and non-infectious inflammation that underlies various inflammatory diseases such as ischemia, atherosclerosis and cancer. The Company of Biologists Ltd 2017-03-01 /pmc/articles/PMC5374318/ /pubmed/28250052 http://dx.doi.org/10.1242/dmm.027102 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Kenmoku, Hiroyuki
Hori, Aki
Kuraishi, Takayuki
Kurata, Shoichiro
A novel mode of induction of the humoral innate immune response in Drosophila larvae
title A novel mode of induction of the humoral innate immune response in Drosophila larvae
title_full A novel mode of induction of the humoral innate immune response in Drosophila larvae
title_fullStr A novel mode of induction of the humoral innate immune response in Drosophila larvae
title_full_unstemmed A novel mode of induction of the humoral innate immune response in Drosophila larvae
title_short A novel mode of induction of the humoral innate immune response in Drosophila larvae
title_sort novel mode of induction of the humoral innate immune response in drosophila larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374318/
https://www.ncbi.nlm.nih.gov/pubmed/28250052
http://dx.doi.org/10.1242/dmm.027102
work_keys_str_mv AT kenmokuhiroyuki anovelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae
AT horiaki anovelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae
AT kuraishitakayuki anovelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae
AT kuratashoichiro anovelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae
AT kenmokuhiroyuki novelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae
AT horiaki novelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae
AT kuraishitakayuki novelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae
AT kuratashoichiro novelmodeofinductionofthehumoralinnateimmuneresponseindrosophilalarvae