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Efficient TALEN-mediated gene targeting of chicken primordial germ cells

In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal species and is posited to control avian germ cell formation. We show that TALENs mediate homology-directed r...

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Detalles Bibliográficos
Autores principales:	Taylor, Lorna, Carlson, Daniel F., Nandi, Sunil, Sherman, Adrian, Fahrenkrug, Scott C., McGrew, Michael J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Company of Biologists Ltd 2017
Materias:	Techniques and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374353/ https://www.ncbi.nlm.nih.gov/pubmed/28174243 http://dx.doi.org/10.1242/dev.145367

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