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Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences
Background: Diabetes mellitus (DM), particularly type 2 DM (T2DM), is one of the most common metabolic disorder worldwide. The prevention measures and treatment strategies for DM are improving steadily. The current study explains the impact of germination on phytochemical content of Thai black rice...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374407/ https://www.ncbi.nlm.nih.gov/pubmed/28036014 http://dx.doi.org/10.3390/ph10010003 |
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author | Chaiyasut, Chaiyavat Sivamaruthi, Bhagavathi Sundaram Pengkumsri, Noppawat Keapai, Waranya Kesika, Periyanaina Saelee, Manee Tojing, Parichart Sirilun, Sasithorn Chaiyasut, Khontaros Peerajan, Sartjin Lailerd, Narissara |
author_facet | Chaiyasut, Chaiyavat Sivamaruthi, Bhagavathi Sundaram Pengkumsri, Noppawat Keapai, Waranya Kesika, Periyanaina Saelee, Manee Tojing, Parichart Sirilun, Sasithorn Chaiyasut, Khontaros Peerajan, Sartjin Lailerd, Narissara |
author_sort | Chaiyasut, Chaiyavat |
collection | PubMed |
description | Background: Diabetes mellitus (DM), particularly type 2 DM (T2DM), is one of the most common metabolic disorder worldwide. The prevention measures and treatment strategies for DM are improving steadily. The current study explains the impact of germination on phytochemical content of Thai black rice (BR), and the influence of germinated BR extract (GBRE) supplementation on diabetic conditions in rats. Methods: BR was germinated and the phenolic, anthocyanin, and γ-aminobutyric acid (GABA) content of the extract were analyzed using HPLC and spectrophotometric methods. Streptozotocin-induced diabetic rats were supplemented with high and low doses of GBRE. The plasma glucose, insulin, cholesterol, triglyceride levels, antioxidant status, and antioxidant enzyme levels of treated animals were assessed using ELISA and spectrophotometric methods. Results: Germination enhanced the GABA content of BR, and GBRE intervention improved the total antioxidant capacity and antioxidant enzymes levels in diabetic rats. The plasma glucose, cholesterol, triglyceride levels, insulin resistance and glucose tolerance were reduced, and the degree of insulin secretion in rat plasma was significantly increased upon GBRE treatment. Both pre and post-treatment approaches showed the anti-diabetic ability of GBRE. In most of the analyzed parameters, GBRE was quite equal to the performance of drug-metformin. Conclusions: GBRE supplementation helps prevent and manage the consequences of DM. |
format | Online Article Text |
id | pubmed-5374407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53744072017-04-10 Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences Chaiyasut, Chaiyavat Sivamaruthi, Bhagavathi Sundaram Pengkumsri, Noppawat Keapai, Waranya Kesika, Periyanaina Saelee, Manee Tojing, Parichart Sirilun, Sasithorn Chaiyasut, Khontaros Peerajan, Sartjin Lailerd, Narissara Pharmaceuticals (Basel) Article Background: Diabetes mellitus (DM), particularly type 2 DM (T2DM), is one of the most common metabolic disorder worldwide. The prevention measures and treatment strategies for DM are improving steadily. The current study explains the impact of germination on phytochemical content of Thai black rice (BR), and the influence of germinated BR extract (GBRE) supplementation on diabetic conditions in rats. Methods: BR was germinated and the phenolic, anthocyanin, and γ-aminobutyric acid (GABA) content of the extract were analyzed using HPLC and spectrophotometric methods. Streptozotocin-induced diabetic rats were supplemented with high and low doses of GBRE. The plasma glucose, insulin, cholesterol, triglyceride levels, antioxidant status, and antioxidant enzyme levels of treated animals were assessed using ELISA and spectrophotometric methods. Results: Germination enhanced the GABA content of BR, and GBRE intervention improved the total antioxidant capacity and antioxidant enzymes levels in diabetic rats. The plasma glucose, cholesterol, triglyceride levels, insulin resistance and glucose tolerance were reduced, and the degree of insulin secretion in rat plasma was significantly increased upon GBRE treatment. Both pre and post-treatment approaches showed the anti-diabetic ability of GBRE. In most of the analyzed parameters, GBRE was quite equal to the performance of drug-metformin. Conclusions: GBRE supplementation helps prevent and manage the consequences of DM. MDPI 2016-12-28 /pmc/articles/PMC5374407/ /pubmed/28036014 http://dx.doi.org/10.3390/ph10010003 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chaiyasut, Chaiyavat Sivamaruthi, Bhagavathi Sundaram Pengkumsri, Noppawat Keapai, Waranya Kesika, Periyanaina Saelee, Manee Tojing, Parichart Sirilun, Sasithorn Chaiyasut, Khontaros Peerajan, Sartjin Lailerd, Narissara Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences |
title | Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences |
title_full | Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences |
title_fullStr | Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences |
title_full_unstemmed | Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences |
title_short | Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences |
title_sort | germinated thai black rice extract protects experimental diabetic rats from oxidative stress and other diabetes-related consequences |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374407/ https://www.ncbi.nlm.nih.gov/pubmed/28036014 http://dx.doi.org/10.3390/ph10010003 |
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