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Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine

BACKGROUND: Atlantic cod (Gadus morhua), is known to vary genetically across the North Atlantic, Greenland, and Newfoundland. This genetic variation occurs both spatially and temporally through decades of heavy fishing, and is concentrated in three linkage disequilibrium blocks, previously defined b...

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Autores principales: Barney, Bryan T., Munkholm, Christiane, Walt, David R., Palumbi, Stephen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374575/
https://www.ncbi.nlm.nih.gov/pubmed/28359300
http://dx.doi.org/10.1186/s12864-017-3660-3
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author Barney, Bryan T.
Munkholm, Christiane
Walt, David R.
Palumbi, Stephen R.
author_facet Barney, Bryan T.
Munkholm, Christiane
Walt, David R.
Palumbi, Stephen R.
author_sort Barney, Bryan T.
collection PubMed
description BACKGROUND: Atlantic cod (Gadus morhua), is known to vary genetically across the North Atlantic, Greenland, and Newfoundland. This genetic variation occurs both spatially and temporally through decades of heavy fishing, and is concentrated in three linkage disequilibrium blocks, previously defined by pedigreed linkage mapping analysis. Variation within these genomic regions is correlated with both seawater temperature and behavioral ecotype. The full extent and nature of these linkage groups is important information for interpreting cod genetic structure as a tool for future fisheries management. RESULTS: We conducted whole genome sequencing for 31 individual cod from three sub-populations in the Gulf of Maine. Across the genome, we found 3,390,654 intermediate to high frequency Single Nucleotide Polymorphisms (SNPs). We show that pairwise linkage analysis among these SNPs is a powerful tool to detect linkage disequilibrium clusters by recovering the three previously detected linkage groups and identifying the 1031 genes contained therein. Across these genes, we found significant population differentiation among spawning groups in the Gulf of Maine and between Georges Bank and Gulf of Maine. Coordinated divergence among these genes and their differentiation at both short and long spatial scales suggests that they are acting as linked supergenes in local adaptation of cod populations. CONCLUSIONS: Differentiation between SNPs in linkage disequilibrium blocks is the major signal of genetic differentiation between all groups tested within the Gulf of Maine. Our data provide a map of genes contained in these blocks, allowing an enhanced search for neutral genetic structure for demographic inference and fisheries modeling. Patterns of selection and the history of populations may be possible to identify in cod using this description of linkage disequilibrium blocks and future data sets to robustly separate neutral and selected genetic markers. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-017-3660-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-53745752017-03-31 Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine Barney, Bryan T. Munkholm, Christiane Walt, David R. Palumbi, Stephen R. BMC Genomics Research Article BACKGROUND: Atlantic cod (Gadus morhua), is known to vary genetically across the North Atlantic, Greenland, and Newfoundland. This genetic variation occurs both spatially and temporally through decades of heavy fishing, and is concentrated in three linkage disequilibrium blocks, previously defined by pedigreed linkage mapping analysis. Variation within these genomic regions is correlated with both seawater temperature and behavioral ecotype. The full extent and nature of these linkage groups is important information for interpreting cod genetic structure as a tool for future fisheries management. RESULTS: We conducted whole genome sequencing for 31 individual cod from three sub-populations in the Gulf of Maine. Across the genome, we found 3,390,654 intermediate to high frequency Single Nucleotide Polymorphisms (SNPs). We show that pairwise linkage analysis among these SNPs is a powerful tool to detect linkage disequilibrium clusters by recovering the three previously detected linkage groups and identifying the 1031 genes contained therein. Across these genes, we found significant population differentiation among spawning groups in the Gulf of Maine and between Georges Bank and Gulf of Maine. Coordinated divergence among these genes and their differentiation at both short and long spatial scales suggests that they are acting as linked supergenes in local adaptation of cod populations. CONCLUSIONS: Differentiation between SNPs in linkage disequilibrium blocks is the major signal of genetic differentiation between all groups tested within the Gulf of Maine. Our data provide a map of genes contained in these blocks, allowing an enhanced search for neutral genetic structure for demographic inference and fisheries modeling. Patterns of selection and the history of populations may be possible to identify in cod using this description of linkage disequilibrium blocks and future data sets to robustly separate neutral and selected genetic markers. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-017-3660-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-03-31 /pmc/articles/PMC5374575/ /pubmed/28359300 http://dx.doi.org/10.1186/s12864-017-3660-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Barney, Bryan T.
Munkholm, Christiane
Walt, David R.
Palumbi, Stephen R.
Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine
title Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine
title_full Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine
title_fullStr Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine
title_full_unstemmed Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine
title_short Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine
title_sort highly localized divergence within supergenes in atlantic cod (gadus morhua) within the gulf of maine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374575/
https://www.ncbi.nlm.nih.gov/pubmed/28359300
http://dx.doi.org/10.1186/s12864-017-3660-3
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