Cargando…
Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase
Understanding the physical mechanisms governing nuclear mechanics is important as it can impact gene expression and development. However, how cell nuclei respond to external cues such as heat is not well understood. Here, we studied the material properties of isolated nuclei in suspension using an o...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374986/ https://www.ncbi.nlm.nih.gov/pubmed/28355535 http://dx.doi.org/10.1016/j.bpj.2017.01.022 |
_version_ | 1782518978090893312 |
---|---|
author | Chan, Chii J. Li, Wenhong Cojoc, Gheorghe Guck, Jochen |
author_facet | Chan, Chii J. Li, Wenhong Cojoc, Gheorghe Guck, Jochen |
author_sort | Chan, Chii J. |
collection | PubMed |
description | Understanding the physical mechanisms governing nuclear mechanics is important as it can impact gene expression and development. However, how cell nuclei respond to external cues such as heat is not well understood. Here, we studied the material properties of isolated nuclei in suspension using an optical stretcher. We demonstrate that isolated nuclei regulate their volume in a highly temperature-sensitive manner. At constant temperature, isolated nuclei behaved like passive, elastic and incompressible objects, whose volume depended on the pH and ionic conditions. When the temperature was increased suddenly by even a few degrees Kelvin, nuclei displayed a repeatable and reversible temperature-induced volume transition, whose sign depended on the valency of the solvent. Such phenomenon is not observed for nuclei subjected to slow heating. The transition temperature could be shifted by adiabatic changes of the ambient temperature, and the magnitude of temperature-induced volume transition could be modulated by modifying the chromatin compaction state and remodeling processes. Our findings reveal that the cell nucleus can be viewed as a highly charged polymer gel with intriguing thermoresponsive properties, which might play a role in nuclear volume regulation and thermosensing in living cells. |
format | Online Article Text |
id | pubmed-5374986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Biophysical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-53749862018-03-28 Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase Chan, Chii J. Li, Wenhong Cojoc, Gheorghe Guck, Jochen Biophys J Nucleic Acids and Genome Biophysics Understanding the physical mechanisms governing nuclear mechanics is important as it can impact gene expression and development. However, how cell nuclei respond to external cues such as heat is not well understood. Here, we studied the material properties of isolated nuclei in suspension using an optical stretcher. We demonstrate that isolated nuclei regulate their volume in a highly temperature-sensitive manner. At constant temperature, isolated nuclei behaved like passive, elastic and incompressible objects, whose volume depended on the pH and ionic conditions. When the temperature was increased suddenly by even a few degrees Kelvin, nuclei displayed a repeatable and reversible temperature-induced volume transition, whose sign depended on the valency of the solvent. Such phenomenon is not observed for nuclei subjected to slow heating. The transition temperature could be shifted by adiabatic changes of the ambient temperature, and the magnitude of temperature-induced volume transition could be modulated by modifying the chromatin compaction state and remodeling processes. Our findings reveal that the cell nucleus can be viewed as a highly charged polymer gel with intriguing thermoresponsive properties, which might play a role in nuclear volume regulation and thermosensing in living cells. The Biophysical Society 2017-03-28 2017-03-28 /pmc/articles/PMC5374986/ /pubmed/28355535 http://dx.doi.org/10.1016/j.bpj.2017.01.022 Text en © 2017 Biophysical Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Nucleic Acids and Genome Biophysics Chan, Chii J. Li, Wenhong Cojoc, Gheorghe Guck, Jochen Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase |
title | Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase |
title_full | Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase |
title_fullStr | Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase |
title_full_unstemmed | Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase |
title_short | Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase |
title_sort | volume transitions of isolated cell nuclei induced by rapid temperature increase |
topic | Nucleic Acids and Genome Biophysics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374986/ https://www.ncbi.nlm.nih.gov/pubmed/28355535 http://dx.doi.org/10.1016/j.bpj.2017.01.022 |
work_keys_str_mv | AT chanchiij volumetransitionsofisolatedcellnucleiinducedbyrapidtemperatureincrease AT liwenhong volumetransitionsofisolatedcellnucleiinducedbyrapidtemperatureincrease AT cojocgheorghe volumetransitionsofisolatedcellnucleiinducedbyrapidtemperatureincrease AT guckjochen volumetransitionsofisolatedcellnucleiinducedbyrapidtemperatureincrease |