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Spatiotemporal development of soaked protein crystal
Crystal soaking is widely performed in biological crystallography. This paper reports time-resolved X-ray crystallographic and microtomographic analyses of tetragonal crystals of chicken egg-white lysozyme soaked in mother liquor containing potassium hexachloroplatinate. The microtomographic analysi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5375977/ https://www.ncbi.nlm.nih.gov/pubmed/25043871 http://dx.doi.org/10.1038/srep05731 |
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author | Mizutani, Ryuta Shimizu, Yusuke Saiga, Rino Ueno, Go Nakamura, Yuki Takeuchi, Akihisa Uesugi, Kentaro Suzuki, Yoshio |
author_facet | Mizutani, Ryuta Shimizu, Yusuke Saiga, Rino Ueno, Go Nakamura, Yuki Takeuchi, Akihisa Uesugi, Kentaro Suzuki, Yoshio |
author_sort | Mizutani, Ryuta |
collection | PubMed |
description | Crystal soaking is widely performed in biological crystallography. This paper reports time-resolved X-ray crystallographic and microtomographic analyses of tetragonal crystals of chicken egg-white lysozyme soaked in mother liquor containing potassium hexachloroplatinate. The microtomographic analysis showed that X-ray attenuation spread from the superficial layer of the crystal and then to the crystal core. The crystallographic analyses indicated that platinum sites can be classified into two groups from the temporal development of the electron densities. A soaking process consisting of binding-rate-driven and equilibrium-driven layers is proposed to describe these results. This study suggests that the composition of chemical and structural species resulting from the soaking process varies depending on the position in the crystal. |
format | Online Article Text |
id | pubmed-5375977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53759772017-04-03 Spatiotemporal development of soaked protein crystal Mizutani, Ryuta Shimizu, Yusuke Saiga, Rino Ueno, Go Nakamura, Yuki Takeuchi, Akihisa Uesugi, Kentaro Suzuki, Yoshio Sci Rep Article Crystal soaking is widely performed in biological crystallography. This paper reports time-resolved X-ray crystallographic and microtomographic analyses of tetragonal crystals of chicken egg-white lysozyme soaked in mother liquor containing potassium hexachloroplatinate. The microtomographic analysis showed that X-ray attenuation spread from the superficial layer of the crystal and then to the crystal core. The crystallographic analyses indicated that platinum sites can be classified into two groups from the temporal development of the electron densities. A soaking process consisting of binding-rate-driven and equilibrium-driven layers is proposed to describe these results. This study suggests that the composition of chemical and structural species resulting from the soaking process varies depending on the position in the crystal. Nature Publishing Group 2014-07-21 /pmc/articles/PMC5375977/ /pubmed/25043871 http://dx.doi.org/10.1038/srep05731 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Article Mizutani, Ryuta Shimizu, Yusuke Saiga, Rino Ueno, Go Nakamura, Yuki Takeuchi, Akihisa Uesugi, Kentaro Suzuki, Yoshio Spatiotemporal development of soaked protein crystal |
title | Spatiotemporal development of soaked protein crystal |
title_full | Spatiotemporal development of soaked protein crystal |
title_fullStr | Spatiotemporal development of soaked protein crystal |
title_full_unstemmed | Spatiotemporal development of soaked protein crystal |
title_short | Spatiotemporal development of soaked protein crystal |
title_sort | spatiotemporal development of soaked protein crystal |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5375977/ https://www.ncbi.nlm.nih.gov/pubmed/25043871 http://dx.doi.org/10.1038/srep05731 |
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