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Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir
Microorganisms play pivotal roles within aquatic ecosystems, affecting their structure, functioning and services. However, little is known about the effects of water stratification and mixing on the aquatic microbial community dynamics in subtropical reservoirs. In this study, we explored vertical a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376048/ https://www.ncbi.nlm.nih.gov/pubmed/25059241 http://dx.doi.org/10.1038/srep05821 |
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author | Yu, Zheng Yang, Jun Amalfitano, Stefano Yu, Xiaoqing Liu, Lemian |
author_facet | Yu, Zheng Yang, Jun Amalfitano, Stefano Yu, Xiaoqing Liu, Lemian |
author_sort | Yu, Zheng |
collection | PubMed |
description | Microorganisms play pivotal roles within aquatic ecosystems, affecting their structure, functioning and services. However, little is known about the effects of water stratification and mixing on the aquatic microbial community dynamics in subtropical reservoirs. In this study, we explored vertical and seasonal patterns of microbial diversity in the Dongzhen Reservoir (southeast China). Quantitative PCR, quantitative RT-PCR, and 454 pyrosequencing were used for an in-depth characterization of the bacterial community across time (every three months for one year) and space (five different water depths). Our results indicated that thermal and oxygen stratification shaped the phylogenetic composition of microbial communities in the reservoir. There were significant differences in physical, chemical and microbiological parameters between epilimnion and hypolimnion (P < 0.05). The RNA: DNA ratios were significantly lower in epilimnion and metalimnion but rapidly increased in hypolimnion (P < 0.05), suggesting that microorganisms were more active at low temperatures, low dissolved oxygen concentrations and high TN/TP ratios. Redundancy analysis and pathway analysis revealed a complex interplay of various environmental and biological factors by explaining the spatiotemporal variations in bacterial communities. Adaptive reservoir management strategies should consider carefully the effects of water stratification and mixing, together with the distribution patterns of aquatic microorganisms. |
format | Online Article Text |
id | pubmed-5376048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53760482017-04-03 Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir Yu, Zheng Yang, Jun Amalfitano, Stefano Yu, Xiaoqing Liu, Lemian Sci Rep Article Microorganisms play pivotal roles within aquatic ecosystems, affecting their structure, functioning and services. However, little is known about the effects of water stratification and mixing on the aquatic microbial community dynamics in subtropical reservoirs. In this study, we explored vertical and seasonal patterns of microbial diversity in the Dongzhen Reservoir (southeast China). Quantitative PCR, quantitative RT-PCR, and 454 pyrosequencing were used for an in-depth characterization of the bacterial community across time (every three months for one year) and space (five different water depths). Our results indicated that thermal and oxygen stratification shaped the phylogenetic composition of microbial communities in the reservoir. There were significant differences in physical, chemical and microbiological parameters between epilimnion and hypolimnion (P < 0.05). The RNA: DNA ratios were significantly lower in epilimnion and metalimnion but rapidly increased in hypolimnion (P < 0.05), suggesting that microorganisms were more active at low temperatures, low dissolved oxygen concentrations and high TN/TP ratios. Redundancy analysis and pathway analysis revealed a complex interplay of various environmental and biological factors by explaining the spatiotemporal variations in bacterial communities. Adaptive reservoir management strategies should consider carefully the effects of water stratification and mixing, together with the distribution patterns of aquatic microorganisms. Nature Publishing Group 2014-07-25 /pmc/articles/PMC5376048/ /pubmed/25059241 http://dx.doi.org/10.1038/srep05821 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Article Yu, Zheng Yang, Jun Amalfitano, Stefano Yu, Xiaoqing Liu, Lemian Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir |
title | Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir |
title_full | Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir |
title_fullStr | Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir |
title_full_unstemmed | Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir |
title_short | Effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir |
title_sort | effects of water stratification and mixing on microbial community structure in a subtropical deep reservoir |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376048/ https://www.ncbi.nlm.nih.gov/pubmed/25059241 http://dx.doi.org/10.1038/srep05821 |
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